Harnessing de novo transcriptome sequencing to identify and characterize genes regulating carbohydrate biosynthesis pathways in Salvia guaranitica L.

Autor:	Abbas ZK; Department of Biology, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia., Al-Huqail AA; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia., Abdel Kawy AH; Plant Ecophysiology Unit, Plant Ecology and Range Management Department, Desert Research Center, Cairo, Egypt., Abdulhai RA; Botany Department, Faculty of Women, Ain Shams University, Cairo, Egypt., Albalawi DA; Department of Biology, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia.; Biodiversity Genomics Unit, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia., AlShaqhaa MA; Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia., Alsubeie MS; Biology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia., Darwish DBE; Department of Biology, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia., Abdelhameed AA; Agricultural Botany Department (Genetics), Faculty of Agriculture, Al-Azhar University, Assuit, Egypt., Soudy FA; Genetics and Genetic Engineering Department, Faculty of Agriculture, Benha University, Moshtohor, Egypt., Makki RM; Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia., Aljabri M; Department of Biology, Faculty of Science, Umm Al-Qura University, Makkah, Saudi Arabia., Al-Sulami N; Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia., Ali M; Maryout Research Station, Genetic Resources Department, Desert Research Center, Cairo, Egypt., Zayed M; Department of Botany and Microbiology, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2024 Sep 26; Vol. 15, pp. 1467432. Date of Electronic Publication: 2024 Sep 26 (Print Publication: 2024).
DOI:	10.3389/fpls.2024.1467432
Abstrakt:	Introduction: Carbohydrate compounds serve multifaceted roles, from energy sources to stress protectants, found across diverse organisms including bacteria, fungi, and plants. Despite this broad importance, the molecular genetic framework underlying carbohydrate biosynthesis pathways, such as starch, sucrose, and glycolysis/gluconeogenesis in Salvia guaranitica , remains largely unexplored. Methods: In this study, the Illumina-HiSeq 2500 platform was used to sequence the transcripts of S. guaranitica leaves, generating approximately 8.2 Gb of raw data. After filtering and removing adapter sequences, 38 million reads comprising 210 million high-quality nucleotide bases were obtained. De novo assembly resulted in 75,100 unigenes, which were annotated to establish a comprehensive database for investigating starch, sucrose, and glycolysis biosynthesis. Functional analyses of glucose-6-phosphate isomerase ( SgGPI ), trehalose-6-phosphate synthase/phosphatase ( SgT6PS ), and sucrose synthase ( SgSUS ) were performed using transgenic Arabidopsis thaliana . Results: Among the unigenes, 410 were identified as putatively involved in these metabolic pathways, including 175 related to glycolysis/gluconeogenesis and 235 to starch and sucrose biosynthesis. Overexpression of SgGPI , SgT6PS , and SgSUS in transgenic A. thaliana enhanced leaf area, accelerated flower formation, and promoted overall growth compared to wild-type plants. Discussion: These findings lay a foundation for understanding the roles of starch, sucrose, and glycolysis biosynthesis genes in S. guaranitica , offering insights into future metabolic engineering strategies for enhancing the production of valuable carbohydrate compounds in S. guaranitica or other plants. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer OH declared a past co-authorship with the author MA, and declared a shared affiliation with the author MZ to the handling editor at the time of the review. (Copyright © 2024 Abbas, Al-Huqail, Abdel Kawy, Abdulhai, Albalawi, AlShaqhaa, Alsubeie, Darwish, Abdelhameed, Soudy, Makki, Aljabri, Al-Sulami, Ali and Zayed.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu