Effect of Stromal Vascular Fraction in the Rat Model of Pharyngocutaneous Fistulas.

Autor:	Durna YM; Otolaryngology, Private Practice, İstanbul, TUR., Yigit O; Ear, Nose, and Throat Diseases, Istanbul Sisli Hamidiye Etfal Research and Training Hospital, İstanbul, TUR., Gül M; Histology and Embryology, İnönü University, Malatya, TUR., Hamit B; Ear Nose and Throat, Ear Nose and Throat Specialist, Private Practice, İstanbul, TUR., Zayman E; Histology and Embryology, Faculty of Medicine, Malatya Turgut Özal University, Malatya, TUR., Demirhan H; Ear, Nose, and Throat Diseases, Faculty of Medicine, Medipol Mega University Hospital, istanbul, TUR., Durna Dastan S; Biology, Faculty of Science, Sivas Cumhuriyet University, Sivas, TUR., Oztel ON; Molecular Biology and Genetics, Iontek Molecular Diagnostics, İstanbul, TUR.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2024 Sep 10; Vol. 16 (9), pp. e69085. Date of Electronic Publication: 2024 Sep 10 (Print Publication: 2024).
DOI:	10.7759/cureus.69085
Abstrakt:	Objective: A pharyngocutaneous fistula is one of the complications after laryngeal and pharyngeal surgery. It may also contribute to wound healing due to adipose tissue-derived stem cells and the growth factors in the stromal vascular fraction. The aim of this study was to investigate the effects of the adipose tissue-derived stromal vascular fraction on wound healing in the pharyngocutaneous fistula model induced in rats. Material and Methods: Approval was received from the Animal Experiments Local Ethics Committee before starting the study (29.01.2016-2016/09). Eleven male Sprague-Dawley rats weighing approximately 300 g were included in the study. The animals were randomly divided into the study and control groups so that each group would include five animals. An animal was assigned as a donor for the removal of omental adipose tissue. Among the animals in which the pharyngocutaneous fistula model was created under general anesthesia, 1 ml of the stromal vascular fraction was injected into the study group on postoperative day 1. In postoperative week 2, all the animals were sacrificed and examined histologically (epithelialization-cell infiltration - mucosal injury). IBM SPSS Statistics for Windows, version 15, was used in the analyses. A p-value <0.05 was considered significant. Results: Epithelialization was higher in the study group compared to the control group. However, no statistically significant difference was found between the two groups (p = 0.08). The cell infiltration was found to be statistically higher in the control group compared to the study group (p = 0.03). The mucosal injury was found to be significantly higher in the control group compared to the study group (p = 0.03). According to the Pearson correlation test, a negative correlation was found between epithelialization and cell infiltration and mucosal injury (p = 0.019 and p = 0.001). A positive correlation was found between cell infiltration and mucosal injury (p = 0.009). Conclusion: It was shown that stromal vascular fraction had a positive effect on wound healing in the pharyngocutaneous fistula model. According to the data we have obtained, we think that it can be effective in the treatment of pharyngocutaneous fistulas after more extensive preclinical and clinical studies are carried out. Competing Interests: Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: Animal Experiments Local Ethics Committee Issued protocol number 29.01.2016-2016/09. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. (Copyright © 2024, Durna et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu