Microbial diversity in primary endodontic infections: demographics and radiographic characteristics.

Autor: Schuweiler D; Division of Endodontics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, 55455, USA., Ordinola-Zapata R; Division of Endodontics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, 55455, USA. rordinol@umn.edu., Dietz M; Division of Basic & Translational Research, Department of Surgery, University of Minnesota, Minneapolis, MN, USA., Lima BP; Division of Basic Sciences, Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, 55455, USA., Noblett WC; Division of Endodontics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, 55455, USA., Staley C; Division of Basic & Translational Research, Department of Surgery, University of Minnesota, Minneapolis, MN, USA.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2024 Oct 11; Vol. 28 (11), pp. 591. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1007/s00784-024-05982-y
Abstrakt: Objective: To analyze if the microbiome community composition in primary endodontic infections is associated with clinical or radiographic factors.
Materials and Methods: Seventy-one patients with primary endodontic infections were evaluated for percussion tenderness, presence of a sinus tract, presence of caries, sex, probing depth > 4 mm, and age. Samples from the root canals were obtained and the microbiome was subsequently characterized by 16 S rRNA amplicon sequencing. For the radiographic analysis, a subset of 12 samples with a periapical index (PAI) ≤ 2 were compared with 19 samples with PAI of 5. The Shannon and Chao1 indices were used to measure alpha diversity. Differences in abundances of genera were evaluated using the Kruskal-Wallis test with Bonferroni's correction. Differences in community composition were evaluated using analysis of similarity (ANOSIM) with Bray-Curtis dissimilarity matrices.
Results: No significant differences in microbiome composition relative to clinical factors were found using ANOSIM. Teeth within the two categories of periapical index showed a similar number of species richness, and alpha diversity values P > 0.05. Community composition was significantly affected by the periapical index (ANOSIM P = 0.039, R = 0.10). Larger radiographic lesions demonstrated significant increase in Prevotellaceae, Olsenella, and the motile bacteria Oribacterium, Selenomonadaceae spp., and Treponema.
Conclusion: Clinical factors associated with apical periodontitis have a limited impact on the root canal microbiome composition. Community composition appears to be affected in teeth with large apical lesions.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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