Stage-specific modulation of multinucleation, fusion, and resorption by the long non-coding RNA DLEU1 and miR-16 in human primary osteoclasts.

Autor: Moura SR; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.; ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal., Sousa AB; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.; ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal., Olesen JB; Department of Pathology, Odense University Hospital, Odense, Denmark.; Clinical Cell Biology, Pathology Research Unit, Department of Clinical Research, University of Southern Denmark, Odense, Denmark.; Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark., Barbosa MA; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.; ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal., Søe K; Department of Pathology, Odense University Hospital, Odense, Denmark.; Clinical Cell Biology, Pathology Research Unit, Department of Clinical Research, University of Southern Denmark, Odense, Denmark.; Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark., Almeida MI; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal. ines.almeida@i3s.up.pt.; INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal. ines.almeida@i3s.up.pt.; ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal. ines.almeida@i3s.up.pt.
Jazyk: angličtina
Zdroj: Cell death & disease [Cell Death Dis] 2024 Oct 11; Vol. 15 (10), pp. 741. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1038/s41419-024-06983-1
Abstrakt: Osteoclasts are the only cells able to resorb all the constituents of the bone matrix. While the modulation of osteoclast activity is well established for preventing bone-related diseases, there is an increasing demand for novel classes of anti-resorption agents. Herein, we investigated non-coding RNA molecules and proposed DLEU1 and miR-16 as potential candidates for modulating osteoclast functions. DLEU1 and miR-16 target cell fusion at both the early and late stages of osteoclastogenesis but operate through independent pathways. DLEU1 silencing hinders the fusion process, leading to abrogation of the phagocytic cup fusion modality and a reduction in the fusion events between mononucleated precursors and multinucleated osteoclasts, while miR-16 influences monocyte-to-osteoclast differentiation, impairing osteoclasts formation but not the number of nuclei at early stages. On the other hand, using these non-coding RNAs to engineer mature osteoclasts has implications for bone resorption. Both DLEU1 and miR-16 influence the speed of resorption in pit-forming osteoclasts, without affecting the resorbed area. However, the impact of increasing miR-16 levels extends more broadly, affecting trench-forming osteoclasts as well, leading to a reduction in their percentage, speed, and resorbed area. These findings offer potential new therapeutic targets to ameliorate bone destruction in skeletal diseases.
(© 2024. The Author(s).)
Databáze: MEDLINE
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