Global characterization of RNA modifying enzymes with RNA-mediated activity-based protein profiling (RNABPP).

Autor:	Yu NJ; Department of Chemistry, Princeton University, Princeton, NJ, United States., Jaber QZ; Department of Chemistry, Princeton University, Princeton, NJ, United States., Kleiner RE; Department of Chemistry, Princeton University, Princeton, NJ, United States. Electronic address: rkleiner@princeton.edu.
Jazyk:	angličtina
Zdroj:	Methods in enzymology [Methods Enzymol] 2024; Vol. 705, pp. 111-125. Date of Electronic Publication: 2024 Aug 10.
DOI:	10.1016/bs.mie.2024.07.010
Abstrakt:	Post-transcriptional RNA modifications can regulate RNA function and play an important role in gene expression. Studying RNA modifying enzymes and their associated modifications remains a considerable challenge. Here we describe the RNA-mediated activity-based protein profiling (RNABPP) methodology, a chemoproteomic strategy for profiling the activity of RNA modifying enzymes in their native context. RNABPP relies upon metabolic RNA labeling with modified ribonucleoside-based probes, combined with protein-RNA enrichment and quantitative proteomics. The RNABPP approach is a general strategy based on chemical reactivity and enzyme mechanism, making it suitable for probing multiple classes of RNA modifying enzymes across diverse biological systems. (Copyright © 2024. Published by Elsevier Inc.)
Databáze:	MEDLINE
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