Microbiological studies on Escherichia coli and Klebsiella pneumoniae causing vaginal and urinary tract inflammation; prevalence, antibiotics-resistance and natural products susceptibility.

Autor: Soliman AM; Botany and Microbiology Department, Faculty of Science, Menoufia University, Egypt., Shaaban MT; Botany and Microbiology Department, Faculty of Science, Menoufia University, Egypt., Dawood AS; Obstetrics and Gynecology Department, Faculty of Medicine, Tanta University, Egypt., Shaheen MN; Botany and Microbiology Department, Faculty of Science, Menoufia University, Egypt., Salama HS; Botany and Microbiology Department, Faculty of Science, Menoufia University, Egypt. Electronic address: husseinsalama@science.menofia.edu.eg.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Nov; Vol. 196, pp. 107000. Date of Electronic Publication: 2024 Oct 09.
DOI: 10.1016/j.micpath.2024.107000
Abstrakt: Great efforts have been made for controlling multidrug-resistant bacteria (MDR). The antibacterial activity of natural products is an effective strategy due to its beneficial effects on human health. This study focused on the isolation, identification, studying the prevalence and the susceptibility of Escherichia coli and Klebsiella spp. to some natural products. After isolation, the isolates primarily identified using biochemical and API tests. Then, the antibiotic susceptibility test was done to reveal the most antibiotics resistant isolates before further identification of Escherichia coli and Klebsiella pneumoniae using phoA and gyrA genes, respectively. Furthermore, the sensitivity of the most antibiotics resistant isolates to apple cider vinegar (ACV), garlic oil (GO), coconut oil (CNO), tea tree oil (TTO), and lavender oil (LO) was estimated. All tested extracts especially ACV and TTO showed good antibacterial activity against MRD selected isolates. Statistical analysis indicated that there was significant difference in ACV and TTO antibacterial response between Escherichia coli and Klebsiella pneumoniae whereas there was no significant difference between natural products activity when tested against Escherichia coli and Klebsiella pneumoniae seperatly. GC-MS spectroscopy analysis revealed the most prominent active constituents present in tested ACV and TTO. Analyzing the 16S rRNA sequence confirmed the two most MRD pathogenic isolates that showed sensitivity to ACV and TTO were Escherichia coli MS 1 and Klebsiella pneumoniae MS 47 , respectively. Essential oils tea tree and apple cider vinegar showed good antibacterial activities against antibiotics-resistant Escherichia coli and Klebsiella pneumoniae causing vaginal and urinary tract inflammation.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Hussein S. Salama reports was provided by Menoufia University. Hussein S. Salama reports a relationship with Menoufia University that includes: employment. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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