Differences in phagosome acidification of Manila clam hemocytes in response to two Perkinsus species with contrasting proliferation dynamics in the host: P. olseni and P. mediterraneus.

Autor: Fuertes VNB; Laboratory of Fish Diseases, Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan., Watanabe Y; Laboratory of Fish Diseases, Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan., Itoh N; Laboratory of Fish Diseases, Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. Electronic address: aitoh-nk@mail.ecc.u-tokyo.ac.jp.
Jazyk: angličtina
Zdroj: Journal of invertebrate pathology [J Invertebr Pathol] 2024 Nov; Vol. 207, pp. 108216. Date of Electronic Publication: 2024 Oct 09.
DOI: 10.1016/j.jip.2024.108216
Abstrakt: Understanding the factors determining the host ranges of Perkinsus spp., a significant group of pathogenic protozoans affecting shellfish, is essential for preventing their spread and designing effective control measures. Considering that differences in the ability to proliferate within the host may influence the determination of host range, we first injected six Perkinsus spp. into Manila clams Ruditapes philippinarum and monitored the variations of trophozoite numbers. Although all six species were detected in the challenged clams 28 days post infection, the infection intensities varied among species, and particularly two species showed contrasting infection trends: P. mediterraneus showed a decreasing trend of infection, declining to the lowest intensity, whereas that of P. olseni continuously increased, reaching the highest intensity. In vitro exposure to Manila clam hemocytes revealed that the survival of P. mediterraneus trophozoites was suppressed, in contrast to P. olseni, which maintained their viability. Despite similar phagocytic indices for both species, the rate of phagosome acidification was significantly higher for hemocytes phagocytizing P. mediterraneus than those targeting P. olseni. Notably, phagosome acidification was significantly suppressed in hemocytes phagocytizing live P. olseni trophozoites, suggesting that P. olseni may secrete a substance that modulates phagosome acidification, and thereby evades intracellular digestion by the host's hemocytes. Conversely, P. mediterraneus, with a lower affinity for infecting Manila clams, did not exhibit such modulation. Based on these results, we consider that the ability to modulate phagosome acidification in host hemocytes might be at least one factor in determining the host range of Perkinsus species.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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