Nanozyme-mediated ratiometric fluorescence hydrogel for on-site detection of sulfite in food.

Autor: Yang SQ; Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou Dublin International College of Life Sciences and Technology, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Jia BZ; College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China; School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand., Liu J; Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou Dublin International College of Life Sciences and Technology, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Wang H; Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou Dublin International College of Life Sciences and Technology, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Lei HT; Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou Dublin International College of Life Sciences and Technology, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Luo L; Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou Dublin International College of Life Sciences and Technology, College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: lin.luo@scau.edu.cn., Xu ZL; Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou Dublin International College of Life Sciences and Technology, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2025 Jan 15; Vol. 463 (Pt 4), pp. 141525. Date of Electronic Publication: 2024 Oct 04.
DOI: 10.1016/j.foodchem.2024.141525
Abstrakt: In this work, a ratiometric fluorescence hydrogel nanosensor was developed by integrating a composite consisting of o-phenylenediamine (OPD), manganese dioxide nanoflakes (MnO 2 NFs), and N-doped carbon dots (N-CDs) into an agarose hydrogel for sulfite detection. MnO 2 NFs demonstrated intense oxidase-like activity, facilitating the conversion of non-fluorescent OPD into yellow-emissive 2,3-diaminophenazine (DAP). As a result, a significant emission peak belongs to DAP, alongside the fluorescence quenching of N-CDs through FRET. Upon interaction with sulfite, MnO 2 NFs lost their oxidase-like function. This process decreased the fluorescence of DAP and restored the blue fluorescence of N-CDs, producing a typical ratiometric response, ranging from 3 nM ∼ 400 μM, with a detection limit (LOD) of 3.79 nM. Employing a smartphone, the fluorescence color change demonstrated by the hydrogel sensor was translated into quantitative data (LOD: 8.44 nM). This hydrogel sensor offers an affordable, portable, and user-friendly solution for sulfite detection and food safety monitoring.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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