Protocol to establish turkey oviductal organoids as an in vitro model.

Autor:	Santativongchai P; Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA., Klaeui CC; Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA., Kosonsiriluk S; Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA., Saqui-Salces M; Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA., Reed KM; Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN 55108, USA., Wileman BW; Select Genetics, Willmar, MN 56201, USA., Studniski MM; Select Genetics, Willmar, MN 56201, USA., Boukherroub KS; Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA. Electronic address: kahina@umn.edu.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Dec 20; Vol. 5 (4), pp. 103384. Date of Electronic Publication: 2024 Oct 09.
DOI:	10.1016/j.xpro.2024.103384
Abstrakt:	The study of reproductive function in turkey hens has been difficult due to the lack of a reliable, representative in vitro model for investigating profound physiological aspects. This article presents a protocol to establish turkey oviductal organoids, including steps for isolating turkey oviduct epithelial cells followed by seeding and maintaining 3D organoid cultures. We also detail procedures for organoid fixation for histological analysis. This organoid model could serve as a valuable in vitro tool for understanding the intricacies of turkey reproductive physiology. Competing Interests: Declaration of interests B.W.W. and M.S. were employed by the company Select Genetics. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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