Emergence and Rapid Diagnosis of Talaromyces marneffei Infections in Renal Transplant Recipients by Next-Generation Sequencing.

Autor: Xing F; Department of Infectious Diseases and Microbiology, The University of Hong Kong - Shenzhen Hospital, Shenzhen, Guangdong, China., Deng C; Department of Infectious Diseases and Microbiology, The University of Hong Kong - Shenzhen Hospital, Shenzhen, Guangdong, China., Zou S; Department of Adult Intensive Care, The University of Hong Kong - Shenzhen Hospital, Shenzhen, Guangdong, China., Tsang CC; School of Medical and Health Sciences, Tung Wah College, Homantin, Hong Kong, China., Lo SKF; Department of Infectious Diseases and Microbiology, The University of Hong Kong - Shenzhen Hospital, Shenzhen, Guangdong, China., Lau SKP; Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China. skplau@hku.hk., Woo PCY; Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China. pcywoo@hku.hk.; Doctoral Program in Translational Medicine and Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan. pcywoo@hku.hk.; The iEGG and Animal Biotechnology Research Center, National Chung Hsing University, Taichung 402, Taiwan. pcywoo@hku.hk.
Jazyk: angličtina
Zdroj: Mycopathologia [Mycopathologia] 2024 Oct 10; Vol. 189 (6), pp. 91. Date of Electronic Publication: 2024 Oct 10.
DOI: 10.1007/s11046-024-00898-3
Abstrakt: In the last few years, next-generation sequencing (NGS) has emerged as a technology for laboratory diagnosis of many culture-negative infections and slow-growing microorganisms. In this study, we describe the use of metagenomic NGS (mNGS) for rapid diagnosis of T. marneffei infection in a 37-year-old renal transplant recipient who presented with chronic pneumonia syndrome. Bronchoalveolar lavage for mNGS was positive for T. marneffei sequence reads. Prolonged incubation of the bronchoalveolar lavage revealed T. marneffei colonies after 6 days of incubation. Analysis of 23 cases of T. marneffei infections in renal transplant recipients from the literature revealed that the number of cases ranged from 1 to 4 cases per five years from 1990 to 2020; but increased rapidly to 9 cases from 2021 to 2023, with 7 of them diagnosed by NGS. Twenty of the 23 cases were from T. marneffei-endemic areas [southern part of mainland China (n = 9); Hong Kong (n = 4); northeastern India (n = 2); Indonesia (n = 1) and Taiwan (n = 4)]. For the 3 patients from non-T. marneffei-endemic areas [United Kingdom (n = 2) and Australia (n = 1)], they had travel histories to China and Vietnam respectively. The time taken for diagnosis by mNGS [median 1 (range 1 to 2) day] was significantly shorter than that for fungal culture [median 6 (range 3 to 15) days] (P = 0.002). mNGS is useful for picking up more cases of T. marneffei infections in renal transplant recipients as well as providing a rapid diagnosis. Talaromycosis is an emerging fungal infection in renal transplant recipients.
(© 2024. The Author(s).)
Databáze: MEDLINE
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