Characterizing the urobiome in geriatric males with chronic indwelling urinary catheters: an exploratory longitudinal study.

Autor:	Stewart E; Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA., Hochstedler-Kramer BR; Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA., Khemmani M; Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA., Clark NM; Division of Infectious Diseases, Loyola University Medical Center, Maywood, Illinois, USA.; Infectious Disease and Immunology Research Institute, Loyola University Chicago, Maywood, Illinois, USA., Parada JP; Division of Infectious Diseases, Loyola University Medical Center, Maywood, Illinois, USA.; Infectious Disease and Immunology Research Institute, Loyola University Chicago, Maywood, Illinois, USA., Farooq A; Department of Urology, Loyola University Medical Center, Maywood, Illinois, USA., Doshi C; Department of Urology, Loyola University Medical Center, Maywood, Illinois, USA., Wolfe AJ; Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA.; Infectious Disease and Immunology Research Institute, Loyola University Chicago, Maywood, Illinois, USA., Albarillo FS; Division of Infectious Diseases, Loyola University Medical Center, Maywood, Illinois, USA.; Infectious Disease and Immunology Research Institute, Loyola University Chicago, Maywood, Illinois, USA.
Jazyk:	angličtina
Zdroj:	Microbiology spectrum [Microbiol Spectr] 2024 Nov 05; Vol. 12 (11), pp. e0094124. Date of Electronic Publication: 2024 Oct 10.
DOI:	10.1128/spectrum.00941-24
Abstrakt:	The impact of chronic indwelling urinary catheters (IUCs) on the composition and stability of the urinary microbiota remains unknown. The primary aim of this study was to describe the urinary microbiomes of geriatric males with chronic IUCs. A secondary aim was to explore clinical catheter-associated urinary tract infection (CAUTI) courses of the participants. Geriatric male patients with chronic IUCs were followed longitudinally. Catheterized urine, catheter tips, and both urethral and periurethral swabs were collected from participants at monthly intervals. Microbes were isolated and identified from each specimen using an enhanced culture method called expanded quantitative urine culture (EQUC) and targeted 16S rRNA gene DNA sequencing. Microbial outcomes were examined both in the absence of urinary symptoms and in the context of clinical diagnosis of CAUTI. Ten male participants (mean age 86 years) were enrolled. Urinary microbiomes differed for each participant. However, within each individual, microbiomes were similar over time and across niches (bladder, catheter, urethra, and periurethra). Within-niche microbiomes differed across individuals, and this was observed over time. The most abundant bacteria isolated from all niches were known uropathogens. Six of 10 individuals met diagnostic criteria for CAUTI at least once during the 12-month observation period, but no evidence of this or antibiotic treatment/response was discernable in our monthly samples. The microbiomes of each participant were unique and remained similar over time and across niches. Longitudinal EQUC or 16S rRNA gene sequencing data could be useful to clinicians when diagnosing or treating possible CAUTI.IMPORTANCECatheter-associated urinary tract infections (CAUTIs) are serious but preventable nosocomial infections. The most common risk factor for developing CAUTI is prolonged use of indwelling urinary catheters (IUCs). This study provides the first longitudinal description of the urinary microbiomes of geriatric males with chronic IUCs, in the absence of urinary signs and symptoms, as a first step toward enhancing our knowledge of the impact of chronic IUCs on the composition and stability of the urinary microbiota. This is an understudied area, particularly for males. Competing Interests: A.J.W. discloses membership on the Scientific Advisory Boards of Urobiome Therapeutics and Pathnostics. He also discloses funding from the Craig H. Neilsen Foundation, Pathnostics, and an anonymous donor. J.P.P. discloses membership on the Shionogi and Innoviva speaker's bureaus. The other authors have no relevant disclosures.
Databáze:	MEDLINE
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