Fluorescent and bioluminescent bovine H5N1 influenza viruses for evaluation of antiviral interventions.

Autor:	Trimarco JD; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA., Spurrier MA; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA., Skavicus S; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA., Luo Z; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA., Dutta M; Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA., Janowska K; Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA., Acharya P; Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA.; Department of Surgery, Duke University School of Medicine, Durham, North Carolina, USA.; Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina, USA., Heaton BE; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA., Heaton NS; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA.; Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA.; Duke Center for Virology, Duke University School of Medicine, Durham, North Carolina, USA.
Jazyk:	angličtina
Zdroj:	Journal of virology [J Virol] 2024 Nov 19; Vol. 98 (11), pp. e0138524. Date of Electronic Publication: 2024 Oct 10.
DOI:	10.1128/jvi.01385-24
Abstrakt:	In early 2024, a clade 2.3.4.4b high pathogenic H5N1 avian influenza virus was detected in dairy cows and humans in the United States. Since then, it has spread to herds in at least 13 states and caused symptomatic disease in at least fifteen people. To facilitate rapid testing of existing and novel countermeasures, here, we report the development of an H5N1 viral reverse genetic system, its use to produce fluorescent and bioluminescent variant strains, and their utility in high-throughput evaluation of antiviral interventions. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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