Lineage tracing of Shh+ floor plate cells and dynamics of dorsal-ventral gene expression in the regenerating axolotl spinal cord.

Autor: Arbanas LI; Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Vienna, Austria.; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Cura Costa E; Institute of Physics of Liquids and Biological Systems (IFLYSIB), National Scientific and Technical Research Council (CONICET), University of La Plata, La Plata, Argentina., Chara O; School of Biosciences, University of Nottingham, Nottingham, UK.; Center for Information Services and High Performance Computing, Technische Universität Dresden, Dresden, Germany.; Instituto de Tecnología, Universidad Argentina de la Empresa, Buenos Aires, Argentina., Otsuki L; Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Vienna, Austria.; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Tanaka EM; Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Vienna, Austria.; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Jazyk: angličtina
Zdroj: Development, growth & differentiation [Dev Growth Differ] 2024 Oct; Vol. 66 (8), pp. 414-425. Date of Electronic Publication: 2024 Oct 10.
DOI: 10.1111/dgd.12945
Abstrakt: Both development and regeneration depend on signaling centers, which are sources of locally secreted tissue-patterning molecules. As many signaling centers are decommissioned before the end of embryogenesis, a fundamental question is how signaling centers can be re-induced later in life to promote regeneration after injury. Here, we use the axolotl salamander model (Ambystoma mexicanum) to address how the floor plate is assembled for spinal cord regeneration. The floor plate is an archetypal vertebrate signaling center that secretes Shh ligand and patterns neural progenitor cells during embryogenesis. Unlike mammals, axolotls continue to express floor plate genes (including Shh) and downstream dorsal-ventral patterning genes in their spinal cord throughout life, including at steady state. The parsimonious hypothesis that Shh+ cells give rise to functional floor plate cells for regeneration had not been tested. Using HCR in situ hybridization and mathematical modeling, we first quantified the behaviors of dorsal-ventral spinal cord domains, identifying significant increases in gene expression level and floor plate size during regeneration. Next, we established a transgenic axolotl to specifically label and fate map Shh+ cells in vivo. We found that labeled Shh+ cells gave rise to regeneration floor plate, and not to other neural progenitor domains, after tail amputation. Thus, despite changes in domain size and downstream patterning gene expression, Shh+ cells retain their floor plate identity during regeneration, acting as a stable cellular source for this regeneration signaling center in the axolotl spinal cord.
(© 2024 The Author(s). Development, Growth & Differentiation published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Developmental Biologists.)
Databáze: MEDLINE
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