Nasal microbionts differentially colonize and elicit cytokines in human nasal epithelial organoids.

Autor:	Boyd AI; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.; These authors contributed equally., Kafer LA; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.; These authors contributed equally., Escapa IF; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA., Kambal A; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA., Tariq H; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA., Hilsenbeck SG; Duncan Cancer Center, Baylor College of Medicine, Houston, Texas, USA., Nguyen-Phuc H; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA., Rajan A; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.; Present address: Department of Medical Sciences and Technology, Indian Institute of Technology, Madras, Chennai, Tamil Nadu, India., Lensmire JM; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.; Present address: Immunartes, Chicago, Illinois., Patras KA; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.; Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, Texas, USA., Piedra PA; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.; Division of Infectious Diseases, Texas Children's Hospital and Department of Pediatrics Baylor College of Medicine, Houston, Texas, USA., Blutt SE; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.; Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, Texas, USA., Lemon KP; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.; Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, Texas, USA.; Division of Infectious Diseases, Texas Children's Hospital and Department of Pediatrics Baylor College of Medicine, Houston, Texas, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Sep 25. Date of Electronic Publication: 2024 Sep 25.
DOI:	10.1101/2024.09.25.614934
Abstrakt:	Nasal colonization by Staphylococcus aureus or Streptococcus pneumoniae is associated with an increased risk of infection by these pathobionts, whereas nasal colonization by Dolosigranulum species is associated with health. Human nasal epithelial organoids (HNOs) physiologically recapitulate human nasal respiratory epithelium with a robust mucociliary blanket. We reproducibly monocolonized HNOs with these three bacteria for up to 48 hours with varying kinetics across species. HNOs tolerated bacterial monocolonization with localization of bacteria to the mucus layer and minimal cytotoxicity compared to uncolonized HNOs. Human nasal epithelium exhibited both species-specific and general cytokine responses, without induction of type I interferons, consistent with colonization rather than infection. Only live S. aureus colonization induced IL-1 family cytokines, suggestive of inflammasome signaling. D. pigrum and live S. aureus decreased CXCL10, whereas S. pneumoniae increased CXCL11, chemokines involved in antimicrobial responses. HNOs are a compelling model system to reveal host-microbe dynamics at the human nasal mucosa. Competing Interests: Declaration of Interests. The authors declare no competing interests.
Databáze:	MEDLINE
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