Comparative transcriptomic rhythms in the mouse and human prefrontal cortex.

Autor:	Burns JN; Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA 15261., Jenkins AK; Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA 15261., Xue X; Department of Biostatistics, University of Pittsburgh, Pittsburgh, PA 15261., Petersen KA; Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA 15261., Ketchesin KD; Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA 15261., Perez MS; Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219.; Department of Human Genetics, School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261., Vadnie CA; David O. Robbins Neuroscience Program, Department of Psychology, Ohio Wesleyan University, Delaware, OH 43015., Scott MR; Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA 15261., Seney ML; Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA 15261., Tseng GC; Department of Biostatistics, University of Pittsburgh, Pittsburgh, PA 15261., McClung CA; Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA 15261.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Sep 26. Date of Electronic Publication: 2024 Sep 26.
DOI:	10.1101/2024.09.26.615154
Abstrakt:	Alterations in multiple subregions of the human prefrontal cortex (PFC) have been heavily implicated in psychiatric diseases. Moreover, emerging evidence suggests that circadian rhythms in gene expression are present across the brain, including in the PFC, and that these rhythms are altered in disease. However, investigation into the potential circadian mechanisms underlying these diseases in animal models must contend with the fact that the human PFC is highly evolved and specialized relative to that of rodents. Here, we use RNA sequencing to lay the groundwork for translational studies of molecular rhythms through a sex-specific, cross species comparison of transcriptomic rhythms between the mouse medial PFC (mPFC) and two subregions of the human PFC, the anterior cingulate cortex (ACC) and the dorsolateral PFC (DLPFC). We find that while circadian rhythm signaling is conserved across species and subregions, there is a phase shift in the expression of core clock genes between the mouse mPFC and human PFC subregions that differs by sex. Furthermore, we find that the identity of rhythmic transcripts is largely unique between the mouse mPFC and human PFC subregions, with the most overlap (20%, 236 transcripts) between the mouse mPFC and the human ACC in females. Nevertheless, we find that basic biological processes are enriched for rhythmic transcripts across species, with key differences between regions and sexes. Together, this work highlights both the evolutionary conservation of transcriptomic rhythms and the advancement of the human PFC, underscoring the importance of considering cross-species differences when using animal models. Competing Interests: Competing interesting The authors have nothing to disclose.
Databáze:	MEDLINE
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