Cataloguing the postnatal small intestinal transcriptome during the period of susceptibility to necrotizing enterocolitis.

Autor:	Oliveira LFS; Division of Newborn Medicine, Boston Children's Hospital, Boston, MA., Wu S; Division of Newborn Medicine, Boston Children's Hospital, Boston, MA., Dasuri VS; Division of Newborn Medicine, Boston Children's Hospital, Boston, MA., Harrington AW; Dept. of Surgery, Johns Hopkins All Children's Hospital, St. Petersburg, FL., Olaloye O; Department of Pediatrics, Yale University School of Medicine, New Haven, CT, USA., Goldsmith J; Dept of Pathology, Boston Children's Hospital, Boston, MA., Breault DT; Division of Endocrinology, Boston Children's Hospital, Boston, MA.; Harvard Stem Cell Institute, Boston, MA.; Dept. of Pediatrics, Harvard Medical School, Boston, MA., Konnikova L; Department of Pediatrics, Yale University School of Medicine, New Haven, CT, USA.; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.; Department of Obstetrics, Gynecology and Reproductive Science, Yale University School of Medicine, New Haven, CT, USA.; Program in Translational Biomedicine, Yale University School of Medicine, New Haven, CT, USA.; Program in Human Translational Immunology, Yale University School of Medicine, New Haven, CT, USA., O'Connell AE; Division of Newborn Medicine, Boston Children's Hospital, Boston, MA.; Dept. of Pediatrics, Harvard Medical School, Boston, MA.; Manton Center for Orphan Disease Research, Boston Children's Hospital, Boston, MA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Sep 27. Date of Electronic Publication: 2024 Sep 27.
DOI:	10.1101/2024.09.25.612672
Abstrakt:	In the first postnatal month, the developing mouse intestine shifts from an immature to a mature intestine that will sustain the organism throughout the lifespan. Here, we surveyed the mouse intestine in C57Bl/6 mice by RNA-Seq to evaluate the changes in gene expression over time from the day of birth through 1 month of age in both the duodenum and ileum. We analyzed gene expression for changes in gene families that correlated with the periods of NEC susceptibility or resistance. We highlight that increased expression of DNA processing genes and vacuolar structure genes, tissue development and morphogenesis genes, and cell migration genes all correlated with NEC susceptibility, while increases in immunity gene sets, intracellular transport genes, ATP production, and intracellular metabolism genes correlated with NEC resistance. Using trends identified in the RNA analyses, we further evaluated expression of cellular markers and epithelial regulators, immune cell markers, and adenosine metabolism components. We confirmed key changes with qRT-PCR and immunofluorescence. In addition, we compared some findings to humans using human intestinal biopsies and organoids. This dataset can serve as a reference for other groups considering the role of single molecules or molecular families in early intestinal and postnatal development. Competing Interests: Disclosures: The authors of this study have no conflicts of interest to declare. Additional Information The authors report no conflicts of interest or competing interests related to this study.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu