Chromosomal integrons are genetically and functionally isolated units of genomes.

Autor:	Blanco P; Molecular Basis of Adaptation, Departamento de Sanidad Animal, Universidad Complutense de Madrid, Madrid 28040, Spain.; VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid 28040, Spain., Trigo da Roza F; Molecular Basis of Adaptation, Departamento de Sanidad Animal, Universidad Complutense de Madrid, Madrid 28040, Spain.; VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid 28040, Spain., Toribio-Celestino L; Departamento de Microbiología Microbiana, Centro Nacional de Biotecnología-CSIC, Madrid 28049, Spain., García-Pastor L; Molecular Basis of Adaptation, Departamento de Sanidad Animal, Universidad Complutense de Madrid, Madrid 28040, Spain.; VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid 28040, Spain., Caselli N; Departamento de Química Física, Universidad Complutense de Madrid, Madrid 28040, Spain., Morón Á; Departamento de Genética, Fisiología y Microbiología, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Madrid 28040, Spain., Ojeda F; Molecular Basis of Adaptation, Departamento de Sanidad Animal, Universidad Complutense de Madrid, Madrid 28040, Spain.; VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid 28040, Spain., Darracq B; Institut Pasteur, Université Paris Cité, CNRS UMR3525, Unité Plasticité du Génome Bactérien, 75015 Paris, France.; Sorbonne Université, ED515, F-75005 Paris, France., Vergara E; Molecular Basis of Adaptation, Departamento de Sanidad Animal, Universidad Complutense de Madrid, Madrid 28040, Spain.; VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid 28040, Spain., Amaro F; Departamento de Genética, Fisiología y Microbiología, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Madrid 28040, Spain., San Millán Á; Departamento de Microbiología Microbiana, Centro Nacional de Biotecnología-CSIC, Madrid 28049, Spain., Skovgaard O; Department of Science and Environment, Roskilde University, 4000 Roskilde, Denmark., Mazel D; Institut Pasteur, Université Paris Cité, CNRS UMR3525, Unité Plasticité du Génome Bactérien, 75015 Paris, France., Loot C; Institut Pasteur, Université Paris Cité, CNRS UMR3525, Unité Plasticité du Génome Bactérien, 75015 Paris, France., Escudero JA; Molecular Basis of Adaptation, Departamento de Sanidad Animal, Universidad Complutense de Madrid, Madrid 28040, Spain.; VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid 28040, Spain.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2024 Nov 11; Vol. 52 (20), pp. 12565-12581.
DOI:	10.1093/nar/gkae866
Abstrakt:	Integrons are genetic elements that increase the evolvability of bacteria by capturing new genes and stockpiling them in arrays. Sedentary chromosomal integrons (SCIs) can be massive and highly stabilized structures encoding hundreds of genes, whose function remains generally unknown. SCIs have co-evolved with the host for aeons and are highly intertwined with their physiology from a mechanistic point of view. But, paradoxically, other aspects, like their variable content and location within the genome, suggest a high genetic and functional independence. In this work, we have explored the connection of SCIs to their host genome using as a model the Superintegron (SI), a 179-cassette long SCI in the genome of Vibrio cholerae N16961. We have relocated and deleted the SI using SeqDelTA, a novel method that allows to counteract the strong stabilization conferred by toxin-antitoxin systems within the array. We have characterized in depth the impact in V. cholerae's physiology, measuring fitness, chromosome replication dynamics, persistence, transcriptomics, phenomics, natural competence, virulence and resistance against protist grazing. The deletion of the SI did not produce detectable effects in any condition, proving that-despite millions of years of co-evolution-SCIs are genetically and functionally isolated units of genomes. (© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu