Pisolithus microcarpus isolates with contrasting abilities to colonise Eucalyptus grandis exhibit significant differences in metabolic signalling.

Autor: Vishwakarma K; Department of Forest Ecology and Management, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden; Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, SE-901 83, Sweden., Buckley S; Department of Forest Ecology and Management, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden., Plett JM; Hawkesbury Institute for the Environment, Western Sydney University, Richmond, NSW, 2753, Australia., Lundberg-Felten J; Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden., Jämtgård S; Department of Forest Ecology and Management, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden; Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, SE-901 83, Sweden. Electronic address: sandra.jamtgard@slu.se., Plett KL; NSW Department of Primary Industries and Regional Development, Elizabeth Macarthur Agricultural Institute, Menangle, NSW, 2568, Australia.
Jazyk: angličtina
Zdroj: Fungal biology [Fungal Biol] 2024 Nov; Vol. 128 (7), pp. 2157-2166. Date of Electronic Publication: 2024 Sep 10.
DOI: 10.1016/j.funbio.2024.09.001
Abstrakt: Biotic factors in fungal exudates impact plant-fungal symbioses establishment. Mutualistic ectomycorrhizal fungi play various ecological roles in forest soils by interacting with trees. Despite progress in understanding secreted fungal signals, dynamics of signal production in situ before or during direct host root contact remain unclear. We need to better understand how variability in intra-species fungal signaling at these stages impacts symbiosis with host tissues. Using the ECM model Pisolithus microcarpus, we selected two isolates (Si9 and Si14) with different abilities to colonize Eucalyptus grandis roots. Hypothesizing that distinct early signalling and metabolite profiles between these isolates would influence colonization and symbiosis, we used microdialysis to non-destructively collect secreted metabolites from either the fungus, host, or both, capturing the dynamic interplay of pre-symbiotic signalling over 48 hours. Our findings revealed significant differences in metabolite profiles between Si9 and Si14, grown alone or with a host root. Si9, with lower colonization efficiency than Si14, secreted a more diverse range of compounds, including lipids, oligopeptides, and carboxylic acids. In contrast, Si14's secretions, similar to the host's, included more aminoglycosides. This study emphasizes the importance of intra-specific metabolomic diversity in ectomycorrhizal fungi, suggesting that early metabolite secretion is crucial for establishing successful mutualistic relationships.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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