Non-enzymatic cholesterol biosensor: Electrochemical sensing based on peptide-polylactic acid thin film.

Autor: Bernardo AL; Biochemistry and Molecular Biology Unit, Department of Bioscience and Technology for Food, Agriculture and Environment - University of Teramo, Campus 'Aurelio Saliceti' Via Renato Balzarini n. 1, 2, 64100 Teramo, Italy. Electronic address: albernardoleonardi@unite.it., Mohammed-Sadhakathullah AHM; Innovation in Materials and Molecular Engineering-Biomaterials for Regenerative Therapies (IMEM-BRT) Group, Department of Chemical Engineering, Universitat Politècnica de Catalunya (UPC), C/ d'Eduard Maristany, 10-14, Building I, 2nd floor, 08019 Barcelona, Spain; Barcelona Research Centre for Multiscale Science and Engineering, Universitat Politècnica de Catalunya (UPC), C/ d'Eduard Maristany, 10-14, Building I, basement, 08019 Barcelona, Spain., Angelucci CB; Department of Veterinary Medicine, University of Teramo, Via Renato Balzarini n.1, 64100 Teramo, Italy., Estrany F; Innovation in Materials and Molecular Engineering-Biomaterials for Regenerative Therapies (IMEM-BRT) Group, Department of Chemical Engineering, Universitat Politècnica de Catalunya (UPC), C/ d'Eduard Maristany, 10-14, Building I, 2nd floor, 08019 Barcelona, Spain; Barcelona Research Centre for Multiscale Science and Engineering, Universitat Politècnica de Catalunya (UPC), C/ d'Eduard Maristany, 10-14, Building I, basement, 08019 Barcelona, Spain., Berghella A; Biochemistry and Molecular Biology Unit, Department of Bioscience and Technology for Food, Agriculture and Environment - University of Teramo, Campus 'Aurelio Saliceti' Via Renato Balzarini n. 1, 2, 64100 Teramo, Italy., Torras J; Barcelona Research Centre for Multiscale Science and Engineering, Universitat Politècnica de Catalunya (UPC), C/ d'Eduard Maristany, 10-14, Building I, basement, 08019 Barcelona, Spain; Department of Veterinary Medicine, University of Teramo, Via Renato Balzarini n.1, 64100 Teramo, Italy., Armelin E; Innovation in Materials and Molecular Engineering-Biomaterials for Regenerative Therapies (IMEM-BRT) Group, Department of Chemical Engineering, Universitat Politècnica de Catalunya (UPC), C/ d'Eduard Maristany, 10-14, Building I, 2nd floor, 08019 Barcelona, Spain; Barcelona Research Centre for Multiscale Science and Engineering, Universitat Politècnica de Catalunya (UPC), C/ d'Eduard Maristany, 10-14, Building I, basement, 08019 Barcelona, Spain. Electronic address: elaine.armelin@upc.edu., Oddi S; Department of Veterinary Medicine, University of Teramo, Via Renato Balzarini n.1, 64100 Teramo, Italy; European Center for Brain Research (CERC), Santa Lucia Foundation I.R.C.C.S., Via del Fosso di Fiorano 64, 00143 Rome, Italy. Electronic address: soddi@unite.it., Dainese E; Biochemistry and Molecular Biology Unit, Department of Bioscience and Technology for Food, Agriculture and Environment - University of Teramo, Campus 'Aurelio Saliceti' Via Renato Balzarini n. 1, 2, 64100 Teramo, Italy. Electronic address: edainese@unite.it.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 281 (Pt 2), pp. 136337. Date of Electronic Publication: 2024 Oct 09.
DOI: 10.1016/j.ijbiomac.2024.136337
Abstrakt: Cholesterol is a fundamental lipid prevalent in eukaryotic cell membranes and circulating in the bloodstream bound to lipoproteins. It serves as a precursor to steroid hormones and is regarded as a biomarker for cardiovascular disease and other metabolic disorders. Numerous cholesterol detection methods predominantly rely on enzymes, which suffer from instability, leading to non-cost-effective biosensors with low sensitivity and poor reusability. Therefore, monitoring cholesterol levels with a feasible, rapid, and stable biosensor is critical for diagnosing and treating various disorders. This study aimed to develop a non-enzymatic cholesterol biosensor based on a selected cholesterol recognition peptide as the detection element. Screen-printed carbon electrodes (SPEs) modified with biocompatible poly-L-lactic acid (PLLA) porous nanomembranes (NMs) were utilized as support for the covalent immobilization of the peptide. Data obtained from electrochemical impedance spectroscopy (EIS) demonstrated the peptide's effective binding affinity towards cholesterol, paving the way for its implementation. The determination of cholesterol with the proposed biosensor exhibited a low limit of detection of 6.31 μM with linear responses ranging from 2-15 μM and 20-40 μM. These findings present an alternative method for cholesterol sensing by integrating novel peptides as biorecognition motifs with biocompatible polymeric materials, potentially useful as biocompatible and future point-of-care sensors.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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