Dietary zinc status is associated with ZnT3 (SLC30A3), IL-6 gene expressions and spinal cord tissue damage in spinal cord tissue in a cuprizone-induced rat Multiple Sclerosis model.

Autor: Bayiroglu AF; Bandirma Onyedi Eylul University, Medical Faculty, Department of Physiology, Bandırma, Turkey., Acar G; Selcuk University, Medical Faculty, Department of Physiology, Konya, Turkey., Gulbahce-Mutlu E; KTO Karatay University, Medical Faculty, Department of Medical Biology, Konya, Turkey., Baltaci SB; İstanbul Medipol University, Medical Faculty, Department of Physiology, İstanbul, Turkey., Mogulkoc R; Selcuk University, Medical Faculty, Department of Physiology, Konya, Turkey., Baltaci AK; Selcuk University, Medical Faculty, Department of Physiology, Konya, Turkey. Electronic address: baltaci@selcuk.edu.tr.
Jazyk: angličtina
Zdroj: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2024 Dec; Vol. 86, pp. 127540. Date of Electronic Publication: 2024 Oct 05.
DOI: 10.1016/j.jtemb.2024.127540
Abstrakt: The aim of this study was to investigate the effects of dietary zinc status on spinal cord tissue damage and ZnT3, IL-6 gene expressions in a cuprizone-induced rat Multiple Sclerosis (MS) model. The study was carried out on 46 adult male rats of the genus Wistar. The animals used in the study were divided into 5 groups (G) (Control 6, other groups 10). G1, Control. G2, Sham-MS: Carboxy-methyl-cellulose (KMS) solution in which Cuprizon was dissolved was given to rats by gavage daily for 8 weeks at the rate of 1 % of daily feed consumption. MS was formed by giving 1 % of the daily feed consumption cuprizon in KMS solution by gavage to the animals in G3, 4 and 5 for 8 weeks. G4 was fed with a zinc deficient (50 µg/kg zinc) diet. G5 was given intraperitoneal (ip) zinc sulfate (5 mg/kg/day) supplementation. MS formation in animals was determined by Rotarod tests and Myelin Basic Protein (MBP) gene expression analysis. ZNT3 and IL-6 gene expression levels in spinal cord tissue samples of animals by Real-Time-PCR method; MDA and GSH levels were determined by ELISA method. The highest spinal cord MDA and IL-6 levels were obtained in G3 and G4 (P<0.05). Zinc supplementation in G5 prevented the increase in the mentioned parameters and turned them into control values (P<0.05). The spinal cord GSH and ZnT3 levels of G3 and G4 were lower than all other groups (P<0.05). Zinc supplementation prevented suppression in the same parameters in G5 and reached the control values (P<0.05). The findings of the current study suggest that zinc supplementation in addition to treatment for MS may be beneficial in reducing the severity of the disease.
Competing Interests: Declaration of Competing Interest The authors declare that they have no potential conficts of interest to disclose.
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Databáze: MEDLINE
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