Convenient rapid prototyping microphysiological niche for mimicking liver native basement membrane: Liver sinusoid on a chip.

Autor: Ebrahimi A; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye., Ghorbanpoor H; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Biomedical Engineering, Eskisehir Osmangazi University, Eskisehir, Türkiye., Apaydın E; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Biochemistry, Institute of Health Sciences, Anadolu University, Eskisehir, Türkiye., Demir Cevizlidere B; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Türkiye., Özel C; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Türkiye., Tüfekçioğlu E; Department of Industrial Design/Department of Industrial Design, Faculty of Architecture and Design, Eskisehir Technical University, Eskisehir, Türkiye., Koç Y; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye., Topal AE; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Biochemistry, School of Pharmacy, Bahçeşehir University, Istanbul, Türkiye., Tomsuk Ö; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Mechanical Engineering, Middle East Technical University, Ankara 06800, Türkiye., Güleç K; Department of Analytical Chemistry, Institute of Health Sciences, Anadolu University, Eskisehir, Türkiye., Abdullayeva N; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Türkiye., Kaya M; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye., Ghorbani A; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Türkiye., Şengel T; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Türkiye; Central Research Laboratory Research and Application Center (ARUM), Eskisehir Osmangazi University, Eskisehir, Türkiye., Benzait Z; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye., Uysal O; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Türkiye., Eker Sarıboyacı A; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Türkiye., Doğan Güzel F; Department of Biomedical Engineering, Ankara Yildirim Beyazit University, Ankara, Türkiye., Singh H; Department of Biological Sciences, Khalifa University, Main Campus, Abu Dhabi, United Arab Emirates; Center for Biotechnology, Khalifa University, Main Campus, Abu Dhabi, United Arab Emirates; Functional Biomaterials Group, Khalifa University, San Campus, Abu Dhabi, United Arab Emirates., Hassan S; Department of Biological Sciences, Khalifa University, Main Campus, Abu Dhabi, United Arab Emirates; Center for Biotechnology, Khalifa University, Main Campus, Abu Dhabi, United Arab Emirates; Functional Biomaterials Group, Khalifa University, San Campus, Abu Dhabi, United Arab Emirates., Ankara H; Mining Engineering Department, Engineering-Architecture Faculty, Eskisehir Osmangazi University, Meşelik Campus, Eskisehir 26480, Türkiye., Pat S; Eskisehir Osmangazi University, Faculty of Science, Department of Physics, Eskisehir TR-26040, Türkiye., Atalay E; Department of Ophthalmology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir 26040, Türkiye., Avci H; Cellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Türkiye; Department of Metallurgical and Materials Engineering, Eskisehir Osmangazi University, Eskisehir, Türkiye; Translational Medicine Research and Clinical Center (TATUM), Eskisehir Osmangazi University, Eskisehir, Türkiye. Electronic address: havci@ogu.edu.tr.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2024 Oct 03; Vol. 245, pp. 114292. Date of Electronic Publication: 2024 Oct 03.
DOI: 10.1016/j.colsurfb.2024.114292
Abstrakt: Liver is responsible for the metabolization processes of up to 90 % of compounds and toxins in the body. Therefore liver-on-a-chip systems, as an in vitro promising cell culture platform, have great importance for fundamental science and drug development. In most of the liver-on-a-chip studies, seeding cells on both sides of a porous membrane, which represents the basement membrane, fail to resemble the native characteristics of biochemical, biophysical, and mechanical properties. In this study, polycarbonate (PC) and polyethylene terephthalate (PET) membranes were coated with gelatin to address this issue by accurately mimicking the native basement membrane present in the space of Disse. Various coating methods were used, including doctor blade, gel micro-injection, electrospinning, and spin coating. Spin coating was demonstrated to be the most effective technique owing to the ability to produce thin gel thickness with desirable surface roughness for cell interactions on both sides of the membrane. HepG2 and EA.HY926 cells were seeded on the upper and bottom sides of the gelatin-coated PET membrane and cultured on-chip for 7 days. Cell viability increased from 90 % to 95 %, while apoptotic index decreased. Albumin secretion notably rose between days 1-7 and 4-7, while GST-α secretion decreased from day 1 to day 7. In conclusion, the optimized spin coating process reported here can effectively modify the membranes to better mimic the native basement membrane niche characteristics.
Competing Interests: Declaration of Competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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