Cortical acetylcholine dynamics are predicted by cholinergic axon activity and behavior state.

Autor:	Neyhart E; Neuroscience Department, Baylor College of Medicine, Houston, TX 77030, USA., Zhou N; Neuroscience Department, Baylor College of Medicine, Houston, TX 77030, USA., Munn BR; Brain and Mind Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia; Complex Systems Group, School of Physics, Faculty of Science, The University of Sydney, Sydney, NSW 2006, Australia., Law RG; Neuroscience Department, Baylor College of Medicine, Houston, TX 77030, USA., Smith C; Neuroscience Department, Baylor College of Medicine, Houston, TX 77030, USA., Mridha ZH; Neuroscience Department, Baylor College of Medicine, Houston, TX 77030, USA., Blanco FA; Neuroscience Department, Baylor College of Medicine, Houston, TX 77030, USA., Li G; State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China; PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China., Li Y; State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing 100871, China; PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China., Hu M; Neuroscience Department, Baylor College of Medicine, Houston, TX 77030, USA., McGinley MJ; Neuroscience Department, Baylor College of Medicine, Houston, TX 77030, USA., Shine JM; Brain and Mind Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia; Complex Systems Group, School of Physics, Faculty of Science, The University of Sydney, Sydney, NSW 2006, Australia., Reimer J; Neuroscience Department, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: reimer@bcm.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2024 Oct 22; Vol. 43 (10), pp. 114808. Date of Electronic Publication: 2024 Oct 08.
DOI:	10.1016/j.celrep.2024.114808
Abstrakt:	Acetylcholine (ACh) is thought to play a role in driving the rapid, spontaneous brain-state transitions that occur during wakefulness; however, the spatiotemporal properties of cortical ACh activity during these state changes are still unclear. We perform simultaneous imaging of GRAB-ACh sensors, GCaMP-expressing basal forebrain axons, and behavior to address this question. We observed a high correlation between axon and GRAB-ACh activity around periods of locomotion and pupil dilation. GRAB-ACh fluorescence could be accurately predicted from axonal activity alone, and local ACh activity decreased at farther distances from an axon. Deconvolution of GRAB-ACh traces allowed us to account for sensor kinetics and emphasized rapid clearance of small ACh transients. We trained a model to predict ACh from pupil size and running speed, which generalized well to unseen data. These results contribute to a growing understanding of the precise timing and spatial characteristics of cortical ACh during fast brain-state transitions. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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