Mercury II -mediated construction of DNA capsules for turn-on fluorescence detection of melamine.

Autor: Singuru MMR; Department of Chemistry, Tunghai University, Taichung, 407224, Taiwan., Chen JL; National Synchrotron Radiation Research Center, Hsinchu, 300092, Taiwan., Chen HY; Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, 807378, Taiwan., Liao WC; Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei, 112304, Taiwan., Chen YY; Department of Chemistry, Tunghai University, Taichung, 407224, Taiwan., Chuang MC; Department of Chemistry, Tunghai University, Taichung, 407224, Taiwan. mcchuang@thu.edu.tw.; Sustainability Science and Management Program, Tunghai University, Taichung, 407224, Taiwan. mcchuang@thu.edu.tw.; International Ph.D. Program in Biomedical & Materials Science, Tunghai University, Taichung, 407224, Taiwan. mcchuang@thu.edu.tw.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Oct 09; Vol. 191 (11), pp. 658. Date of Electronic Publication: 2024 Oct 09.
DOI: 10.1007/s00604-024-06735-0
Abstrakt: Researchers have shown significant interest in three-dimensional DNA building blocks due to their potential applications in biomedicine and biosensing. This study focuses on the synthesis of an Hg II ion-stabilized DNA capsule with T-Hg II -T pairs for the purpose of detecting melamine (MA). MA reacts with Hg II to form a MA-Hg II -MA complex, which causes Hg II to leave the capsule shell, ultimately leading to capsule collapse and release of fluorescent cargo as output signal. Density functional theory (DFT) calculations and X-ray absorption spectroscopy (XAS) were used to demonstrate the ability of MA to extract Hg II from the T-Hg II -T adducts. The DNA capsules were characterized using TEM, SEM, DLS, zeta-potential, and melting curve analysis, which indicated the successful construction of the Hg II -intercalated DNA shell. The MA-triggered destruction of the DNA capsules was visualized by confocal microscopy, and the dynamics of decapsulation were evaluated through fluorescent cargo release. The Hg II -stabilized DNA capsules enable MA detection with a detection limit of 0.037 µM and are insensitive to potential interfering ions and amino acids. The tests conducted using MA spiked milk solution resulted in recoveries ranging from 109 to 113% (0.1 µM) and 94.5 to 96% (0.5 µM). These results suggest that the system is promising for highly accurate and reproducible monitoring of MA adulteration.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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