Lesional Psoriasis is Associated With Alterations in the Stratum Corneum Ceramide Profile and Concomitant Decreases in Barrier Function.

Autor: Rousel J; Centre for Human Drug Research, Leiden, The Netherlands.; Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands., Mergen C; Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands., Bergmans ME; Centre for Human Drug Research, Leiden, The Netherlands.; Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands., Klarenbeek NB; Centre for Human Drug Research, Leiden, The Netherlands., der Kolk TN; Centre for Human Drug Research, Leiden, The Netherlands., van Doorn MBA; Centre for Human Drug Research, Leiden, The Netherlands.; Department of Dermatology, Erasmus Medical Centre, Rotterdam, The Netherlands., Bouwstra JA; Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands., Rissmann R; Centre for Human Drug Research, Leiden, The Netherlands.; Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.; Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: Experimental dermatology [Exp Dermatol] 2024 Oct; Vol. 33 (10), pp. e15185.
DOI: 10.1111/exd.15185
Abstrakt: Psoriasis is an inflammatory skin disease associated with an impaired skin barrier. The skin barrier function is dependent on the extracellular lipid matrix which surrounds the corneocytes in the stratum corneum. Ceramides comprise essential components of this matrix. Alterations in the stratum corneum ceramide profile have been directly linked to barrier dysfunction and might be an underlying factor of the barrier impairment in psoriasis. In this study, we investigated the ceramide profile and barrier function in psoriasis. Lesional and non-lesional skin of 26 patients and 10 healthy controls were analysed using in-depth ceramide lipidomics by liquid chromatography-mass spectrometry. Barrier function was assessed by measuring transepidermal water loss. Lesional skin showed a significant decrease in the abundance of total ceramides with significant alterations in the ceramide subclass composition compared to control and non-lesional skin. Additionally, the percentage of monounsaturated ceramides was significantly increased, and the average ceramide chain length significantly decreased in lesional skin. Altogether, this resulted in a markedly different profile compared to controls for lesional skin, but not for non-lesional skin. Importantly, the reduced barrier function in lesional psoriasis correlated to alterations in the ceramide profile, highlighting their interdependence. By assessing the parameters 2 weeks apart, we are able to highlight the reproducibility of these findings, which further affirms this connection. To conclude, we show that changes in the ceramide profile and barrier impairment are observed in, and limited to, lesional psoriatic skin. Their direct correlation provides a further mechanistic basis for the concomitantly observed impairment of barrier dysfunction.
(© 2024 The Author(s). Experimental Dermatology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: