In vivo volumetric analysis of retinal vascular hemodynamics in mice with spatio-temporal optical coherence tomography.

Autor:	Węgrzyn P; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland.; University of Warsaw, Faculty of Physics, Warsaw, Poland., Kulesza W; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland., Wielgo M; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland., Tomczewski S; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland., Galińska A; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland., Bałamut B; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland., Kordecka K; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland., Cetinkaya O; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland., Foik A; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland., Zawadzki RJ; University of California Davis, Department of Ophthalmology and Vision Science, Sacramento, California, United States., Borycki D; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland., Wojtkowski M; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland.; Nicolaus Copernicus University, Faculty of Physics, Astronomy and Informatics, Toruń, Poland., Curatolo A; International Centre for Translational Eye Research, Warsaw, Poland.; Polish Academy of Sciences, Institute of Physical Chemistry, Warsaw, Poland.; Politecnico di Milano, Department of Physics, Milan, Italy.
Jazyk:	angličtina
Zdroj:	Neurophotonics [Neurophotonics] 2024 Oct; Vol. 11 (4), pp. 0450031-4500322. Date of Electronic Publication: 2024 Oct 08.
DOI:	10.1117/1.NPh.11.4.045003
Abstrakt:	Significance: Microcirculation and neurovascular coupling are important parameters to study in neurological and neuro-ophthalmic conditions. As the retina shares many similarities with the cerebral cortex and is optically accessible, a special focus is directed to assessing the chorioretinal structure, microvasculature, and hemodynamics of mice, a vital animal model for vision and neuroscience research. Aim: We aim to introduce an optical imaging tool enabling in vivo volumetric mouse retinal monitoring of vascular hemodynamics with high temporal resolution. Approach: We translated the spatio-temporal optical coherence tomography (STOC-T) technique into the field of small animal imaging by designing a new optical system that could compensate for the mouse eye refractive error. We also developed post-processing algorithms, notably for the assessment of (i) localized hemodynamics from the analysis of pulse wave-induced Doppler artifact modulation and (ii) retinal tissue displacement from phase-sensitive measurements. Results: We acquired high-quality, in vivo volumetric mouse retina images at a rate of 113 Hz over a lateral field of view of ∼ 500    μ m . We presented high-resolution en face images of the retinal and choroidal structure and microvasculature from various layers, after digital aberration correction. We were able to measure the pulse wave velocity in capillaries of the outer plexiform layer with a mean speed of 0.35 mm/s and identified venous and arterial pulsation frequency and phase delay. We quantified the modulation amplitudes of tissue displacement near major vessels (with peaks of 150 nm), potentially carrying information about the biomechanical properties of the retinal layers involved. Last, we identified the delays between retinal displacements due to the passing of venous and arterial pulse waves. Conclusions: The developed STOC-T system provides insights into the hemodynamics of the mouse retina and choroid that could be beneficial in the study of neurovascular coupling and vasculature and flow speed anomalies in neurological and neuro-ophthalmic conditions. (© 2024 The Authors.)
Databáze:	MEDLINE
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