| Autor: |
Su L; National University of Singapore (Suzhou) Research Institute, 377 Linquan Street, Suzhou, Jiangsu 215123, China.; Department of Food Science and Technology, National University of Singapore, 2 Science Drive 2, Singapore 117542, Singapore., Jing L; National University of Singapore (Suzhou) Research Institute, 377 Linquan Street, Suzhou, Jiangsu 215123, China., Zeng S; National University of Singapore (Suzhou) Research Institute, 377 Linquan Street, Suzhou, Jiangsu 215123, China., Fu C; National University of Singapore (Suzhou) Research Institute, 377 Linquan Street, Suzhou, Jiangsu 215123, China., Huang D; National University of Singapore (Suzhou) Research Institute, 377 Linquan Street, Suzhou, Jiangsu 215123, China.; Department of Food Science and Technology, National University of Singapore, 2 Science Drive 2, Singapore 117542, Singapore. |
| Abstrakt: |
Cultured meat (CM) has been hailed as a sustainable future meat production technology that requires scaffolds to support cell growth. Plant proteins are the most promising raw materials for edible scaffolds but remain underutilized. In this study, kafirin, an abundant, readily available, and nonallergenic prolamin extracted from red sorghum, was explored to fabricate 3D porous sponge-like scaffolds via a simple template-leaching method. The scaffolds featured fully interconnected pores with a high porosity of approximately 84% and mechanical properties of 1.0-1.9 kPa. Porcine skeletal muscle cells (PSCs) and adipose-derived stem cells (ADSCs) could adhere, proliferate, and differentiate on protein scaffolds. Thereafter, a hybrid CM was produced by culturing porcine ADSCs on kafirin scaffolds for 12 days, integrating plant protein-based and cell-based alternatives. The anthocyanins found in red sorghum contributed to the hybrid CM with meat-like color and antioxidative benefits. Moreover, the hybrid CM prototype demonstrated promising potential in providing higher protein content (22.9%) and unique mouthfeel and appearance characteristics, highlighting the viability of sorghum prolamin in promoting CM production. |
