Lactoferrin modulates oxidative stress and inflammatory cytokines in a murine model of dysbiosis induced by clindamycin.

Autor:	Abad I; Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, Spain., Bellés A; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, Spain.; Departamento de Farmacología, Fisiología y Medicina Legal y Forense, Facu1ltad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain., Rodríguez-Largo A; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, Spain.; Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain., Luján L; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, Spain.; Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain., de Blas I; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, Spain.; Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain., Graikini D; Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, Spain., Grasa L; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, Spain.; Departamento de Farmacología, Fisiología y Medicina Legal y Forense, Facu1ltad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.; Instituto de Investigación Sanitaria de Aragón (IIS Aragón), Zaragoza, Spain., Sánchez L; Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, Spain.
Jazyk:	angličtina
Zdroj:	Biochemistry and cell biology = Biochimie et biologie cellulaire [Biochem Cell Biol] 2024 Oct 08. Date of Electronic Publication: 2024 Oct 08.
DOI:	10.1139/bcb-2024-0087
Abstrakt:	Antibiotics, specifically clindamycin (Clin), cause intestinal dysbiosis, reducing the microbiota with anti-inflammatory properties. Furthermore, Clin can induce alterations in the immune responses and oxidative stress. Lactoferrin, among other activities, participates in the maintenance of intestinal homeostasis and reduces dysbiosis induced by antibiotic treatment. The aim of this study was to analyze the effect of native and iron-saturated bovine LF in a murine model of dysbiosis induced by Clin. Six groups of male C57BL/6 mice were treated with saline (control), Clin, native lactoferrin (nLF), iron-saturated lactoferrin (sLF), nLF/Clin, or sLF/Clin. Oxidation caused in the intestinal cells of the ileum of animals subjected to different treatments was analyzed, focusing on lipid peroxidation and protein carbonyl content. The expression of inflammatory mediators was determined by qRT-PCR. Treatment with Clin did not modify lipid peroxidation, but significantly increased protein carbonyl levels up to almost 5-fold respect to the control, an effect that was reversed by orally administering sLF to mice. Furthermore, Clin increased the expression of interleukin-6 and TNF-α by 1- and 2-fold change, respectively. This effect was reversed by treatment with nLF and sLF, decreasing the expression to basal levels. In conclusion, this study indicates that lactoferrin can prevent some of the effects of Clin on intestinal cells and their associated immune system. Competing Interests: The authors declare there are no competing interests.
Databáze:	MEDLINE
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