Inhibition of intestinal inflammation and fibrosis by Scutellaria Baicalensis georgi and Boswellia serrata in human epithelial cells and fibroblasts.

Autor: Laudadio I; Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 324, Rome, Italy., Leter B; Department of Maternal Infantile and Urological Sciences, Sapienza University of Rome, Viale del Policlinico 155, Rome, Italy., Palone F; Laboratory of Biomedical Technologies, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Via Anguillarese, 301, Santa Maria di Galeria Rome, Italy., Cucchiara S; Department of Maternal Infantile and Urological Sciences, Sapienza University of Rome, Viale del Policlinico 155, Rome, Italy., Carissimi C; Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 324, Rome, Italy., Scafa N; Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 324, Rome, Italy., Secci D; Department of Chemistry and Pharmaceutical Technologies, Sapienza University of Rome, P.le Aldo Moro, Rome, Italy., Vitali R; Department of Maternal Infantile and Urological Sciences, Sapienza University of Rome, Viale del Policlinico 155, Rome, Italy., Stronati L; Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 324, Rome, Italy.
Jazyk: angličtina
Zdroj: Immunity, inflammation and disease [Immun Inflamm Dis] 2024 Oct; Vol. 12 (10), pp. e70036.
DOI: 10.1002/iid3.70036
Abstrakt: Objective and Rationale: Inflammatory bowel disease, including Crohn's disease and ulcerative colitis, manifests with chronic intestinal inflammation and frequent sequential fibrosis. Current pharmacological therapies may show harmful side effects and are not useful for prevention or resolution of fibrosis. Thus, the use of alternative therapies is emerging as a novel useful approach. Previous results suggest that Scutellaria baicalensis Georgi (SBG) and Boswellia serrata (BS) display anti-inflammatory properties. The aim of this study was to investigate in intestinal epithelial cells and fibroblasts the anti-inflammatory and anti-fibrotic potential of SBG and BS, alone or in combination.
Methods: Human colorectal adenocarcinoma cells (HT29), human intestinal epithelial cells (HIEC6) and human colon fibroblasts (CCD-18Co) were used. Cells were pretreated with SBG and BS and then exposed to pro-inflammatory and pro-fibrotic cytokines.
Results: SBG and BS extracts significantly decreased pro-inflammatory cytokine expression and improved epithelial restitution in HT29 and HIEC6 cells. Besides, fibrotic marker expression, including SNAIL, ACTA2, ZNF281, was strongly reduced. Colon myofibroblasts treated with SBG and BS showed a significant decrease of fibrotic markers as well.
Conclusions: SBG and BS extracts significantly reduce inflammation and impair fibrosis in intestinal epithelial cells and colon myofibroblasts. No cooperative effect is observed.
(© 2024 The Author(s). Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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