Investigation into the significant role of dermal-epidermal interactions in skin ageing utilising a bioengineered skin construct.

Autor: Costello L; Department of Biosciences, Durham University, Durham, UK., Goncalves K; Department of Biosciences, Durham University, Durham, UK., De Los Santos Gomez P; Department of Biosciences, Durham University, Durham, UK., Hulette B; Mason Business & Innovation Center, The Procter and Gamble Company, Ohio, USA., Dicolandrea T; Mason Business & Innovation Center, The Procter and Gamble Company, Ohio, USA., Flagler MJ; Mason Business & Innovation Center, The Procter and Gamble Company, Ohio, USA., Isfort R; Mason Business & Innovation Center, The Procter and Gamble Company, Ohio, USA., Oblong J; Mason Business & Innovation Center, The Procter and Gamble Company, Ohio, USA., Bascom C; Mason Business & Innovation Center, The Procter and Gamble Company, Ohio, USA., Przyborski S; Department of Biosciences, Durham University, Durham, UK.; Reprocell Europe, Glasgow, UK.
Jazyk: angličtina
Zdroj: Journal of cellular physiology [J Cell Physiol] 2024 Oct 08, pp. e31463. Date of Electronic Publication: 2024 Oct 08.
DOI: 10.1002/jcp.31463
Abstrakt: Increased prevalence of skin ageing is a growing concern due to an ageing global population and has both sociological and psychological implications. The use of more clinically predictive in vitro methods for dermatological research is becoming commonplace due to initiatives and the cost of clinical testing. In this study, we utilise a well-defined and characterised bioengineered skin construct as a tool to investigate the cellular and molecular dynamics involved in skin ageing from a dermal perspective. Through incorporation of ageing fibroblasts into the dermal compartment we demonstrate the significant impact of dermal-epidermal crosstalk on the overlying epidermal epithelium. We characterise the paracrine nature of dermal-epidermal communication and the impact this has during skin ageing. Soluble factors, such as inflammatory cytokines released as a consequence of senescence associated secretory phenotype (SASP) from ageing fibroblasts, are known to play a pivotal role in skin ageing. Here, we demonstrate their effect on epidermal morphology and thickness, but not keratinocyte differentiation or tissue structure. Through a novel in vitro strategy utilising bioengineered tissue constructs, this study offers a unique reductionist approach to study epidermal and dermal compartments in isolation and tandem.
(© 2024 The Author(s). Journal of Cellular Physiology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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