Magnetic resonance imaging classification in a percutaneous needle injury rat model of intervertebral disc degeneration.

Autor: Liang S; Center for Translational Medicine Research and Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China.; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong, China.; Department of Spine and Spinal Cord, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China., Li N; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong, China.; Department of Spine and Spinal Cord, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China., Zhan J; Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing, 100102, China., Li Z; Center for Translational Medicine Research and Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China.; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong, China.; Department of Spine and Spinal Cord, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China., Tie C; Paul C. Lauterbur Research Center for Biomedical lmaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China., Zhu Y; Paul C. Lauterbur Research Center for Biomedical lmaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China., Guo H; CapitalBio Corporation, Beijing, 102206, China., Ke L; Center for Translational Medicine Research and Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China., Li J; Center for Translational Medicine Research and Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China., Xu Z; Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong, China.; Department of Spine and Spinal Cord, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, Shandong, China., Zhang P; Center for Translational Medicine Research and Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China. peng.zhang@siat.ac.cn.; Faculty of Biomedical Engineering, Shenzhen University of Advanced Technology, Shenzhen, 518055, Guangdong, China. peng.zhang@siat.ac.cn.; Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, Shenzhen, 518000, Guangdong, China. peng.zhang@siat.ac.cn.; Shandong Zhongke Advanced Technology Co., Ltd, Jinan, 250300, Shandong, China. peng.zhang@siat.ac.cn., Cheng W; Center for Translational Medicine Research and Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China. wx.cheng@siat.ac.cn.
Jazyk: angličtina
Zdroj: Journal of orthopaedic surgery and research [J Orthop Surg Res] 2024 Oct 07; Vol. 19 (1), pp. 632. Date of Electronic Publication: 2024 Oct 07.
DOI: 10.1186/s13018-024-05110-2
Abstrakt: Background: During the development of disease-modifying intervertebral disc degeneration (IDD) drugs, the rat model of IDD is frequently used for disease progression assessment. The aim of this study was to describe a magnetic resonance (MRI) scoring system for the assessment of different disc conditions in puncture-induced IDD, allowing standardization and comparison of results obtained by different investigators.
Methods: A total of 36 Sprague-Dawley rats were utilized in the present study. The animals were divided into two groups: a sham group and an IDD group caused by puncture. The rats in the IDD group were subsequently divided into six categories based on time frames, with five rats in each category. The sham group was divided into two sub-groups (n = 3) for 28 and 56 days, respectively. T2-weighted images of rats consecutively studied with MRI of the coccygeal discs were classified according to the time course using the corresponding histological data. Additional scoring of the micro-CT was employed to identify the progression of bone destruction of the rat model of IDD.
Results: A comparison of the MRI results between the sham group and the IDD group revealed a significant reduction in NP height, area, T2WI value, and DHI in the latter group (P < 0.05). The micro-CT results demonstrated that following acupuncture, there was a notable decline in the BV, Tb.N, and height of the coccygeal vertebra, while the BS/BV and Tb.Sp exhibited a significant increase (P < 0.05). The histological results were analogous to the MRI results, indicating a progressive exacerbation of IDD and a corresponding increase in NP score (P < 0.05). The results of the MRI were found to be consistent with those of the micro-CT and histological analyses (P < 0.05). The results of the study demonstrate a robust correlation between MRI analysis and histological findings. Live animals are employed for MRI analysis to improve experiment comparability. The reliability of the MRI scoring system ensures assessment of disease progression in live animals, while promoting cost savings and animal welfare by avoiding the sacrifice of animals at different times.
Conclusions: The described scoring paradigm has quantitatively been found to differentiate IDD disease progression in an in vivo rat model. Hence, we suggest employing it to evaluate the rat IDD model and assess the effects of treatments in this model.
(© 2024. The Author(s).)
Databáze: MEDLINE
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