De-novo transcriptome of anterior epimorphic regeneration in Perionyx excavatus.

Autor: Rossan Mathews MG; Regeneration and Stem Cell Biology lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India., Selvan Christyraj JRS; Regeneration and Stem Cell Biology lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India. johnnbt@sathyabama.ac.in., Subramaniam R; Regeneration and Stem Cell Biology lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India., Venkatachalam S; Regeneration and Stem Cell Biology lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India., Selvan Christyraj JD; Regeneration and Stem Cell Biology lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India., Yesudhason BV; Regeneration and Stem Cell Biology lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India. berylvedha.irc@sathyabama.ac.in., Venkatachalam K; Regeneration and Stem Cell Biology lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India., Anandharaj JL; Regeneration and Stem Cell Biology lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India.
Jazyk: angličtina
Zdroj: Scientific data [Sci Data] 2024 Oct 07; Vol. 11 (1), pp. 1093. Date of Electronic Publication: 2024 Oct 07.
DOI: 10.1038/s41597-024-03941-6
Abstrakt: Perionyx excavatus, an indigenous earthworm possesses exceptional regeneration capacity. Their anterior regeneration features wound closure, regeneration induction and morphogenesis of damaged organs. This study involved a complete analysis of their transcriptomic dataset, with an emphasis on identifying the genes expressed during regeneration and predicting their implications in the process of regeneration and morphogenesis. Control (first ten segments) and test (5 th day blastema) RNA samples of biological replicates were isolated and sequenced on NovaSeq. 6000 using PE150 read length. An average of 98.64% of high-quality data was retained with assembly showing better continuity with the average transcript length with 823 bp and N50 value of 1,858 bp. This is the first report on the comparative transcriptome of P. excavatus during anterior regeneration and this study will shed light on the complexity of annelid regeneration.
(© 2024. The Author(s).)
Databáze: MEDLINE
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