A new algorithm for coeliac disease based on the 'long forgotten' TCRγδ + intra-epithelial lymphocytes detected with an antibody working on FFPE sections.

Autor: Kozan EN; Department of Pathology, Ankara University Medical School, Ankara, Turkey.; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA., Kırmızı BA; Department of Pathology, Ankara University Medical School, Ankara, Turkey., Kirsaclioglu CT; Department of Pediatric Gastroenterology, Ankara University Medical School, Ankara, Turkey., Gokmen D; Department of Biostatistics, Ankara University Medical School, Ankara, Turkey., Savas B; Department of Pathology, Ankara University Medical School, Ankara, Turkey., Kansu A; Department of Pediatric Gastroenterology, Ankara University Medical School, Ankara, Turkey., Soykan AI; Department of Gastroenterology, Ankara University Medical School, Ankara, Turkey., Ensari A; Department of Pathology, Ankara University Medical School, Ankara, Turkey.
Jazyk: angličtina
Zdroj: Histopathology [Histopathology] 2024 Oct 07. Date of Electronic Publication: 2024 Oct 07.
DOI: 10.1111/his.15330
Abstrakt: Aims: Diagnosis of coeliac disease (CD) with mild mucosal changes is difficult for all parties involved. We aimed to determine the power of T cell receptor (TCR)γδ + intra-epithelial lymphocytes (IELs) in discriminating CD from other causes of intra-epithelial lymphocytosis using a new monoclonal antibody.
Methods: A total of 167 cases categorised as coeliac (117 untreated CD, classified according to Marsh, updated by Ensari, including 29 type 1, 29 type 2, 39 type 3 and 20 treated CD), and non-coeliac groups (24 controls and 26 non-coeliac IELosis) based on clinical, serological and histological data were studied for IEL counts enumerated per 100 enterocytes using haematoxylin and eosin, CD3, TCR δ-stains.
Results: TCRγδ + IELs were significantly higher in CD (24.83 ± 16.13) compared to non-CD (6.72 ± 6.32) and were correlated with the degree of mucosal damage. Both γδ + IEL count and ratio showed higher performance in differentiating untreated coeliacs from controls, with a sensitivity of 83.76; 85.57 and specificity of 95.83; 79.17, respectively. TCRγδ + IEL counts distinguished type 1 CD (20.41 ± 13.57) from non-coeliac IELosis (9.42 ± 7.28) (p = 0.025). Discriminant analysis revealed that villus/crypt ratio, γδ + and CD3 + IEL counts, γδ + /CD3 + IEL ratio, IEL distribution pattern were potent discriminants and correctly classified 82.3% of cases while the algorithm accurately diagnosed 93.4% of cases.
Conclusions: The new antibody detecting γδ + IELs in FFPE sections revealed thresholds of 10.5 for γδ + IELs and 14% for γδ + /CD3 + IEL ratio which distinguished coeliacs from non-coeliacs with high sensitivity and specificity, particularly in cases with normal villus/crypt axis including type 1 CD, non-CD IELosis and controls. A 'coeliac algorithm' based on γδ + IELs is proposed with the hope that it will be used in the histopathological diagnostic approach by the pathology community.
(© 2024 The Author(s). Histopathology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: