Design and application of microfluidics in aptamer SELEX and Aptasensors.

Autor: Zhang S; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Zhang Y; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Ning Z; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Duan M; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Lin X; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Duan N; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China., Wang Z; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China., Wu S; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China. Electronic address: wusj1986@163.com.
Jazyk: angličtina
Zdroj: Biotechnology advances [Biotechnol Adv] 2024 Dec; Vol. 77, pp. 108461. Date of Electronic Publication: 2024 Oct 05.
DOI: 10.1016/j.biotechadv.2024.108461
Abstrakt: Aptamers are excellent recognition molecules obtained from systematic evolution of ligands by exponential enrichment (SELEX) that have been extensively researched for constructing aptasensors. However, in the process from SELEX to the construction of aptasensors, there are many disadvantages, such as tedious and repetitive operations, interference from external factors, and low efficiency, which seriously limits their application scope and development. Introducing the microfluidic technology can realize the integration and intelligence of SELEX and aptasensing, improve the efficiency of SELEX, and enhance the detection performance and convenience of aptasensing. Hence, in this review, the characteristics of various chips based on different driving forces are described firstly. And then summarizing the design of microfluidic devices based on different SELEX methods and showing the strategies of microfluidic aptasensors based on different detection modes. Finally, discussing the difficulties and challenges encountered when microfluidic is integrated with the SELEX and the aptasensors.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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