Enzyme Inhibitory Activities and RP-HPLC Analysis of Geranium and Erodium Species.

Autor: Özüpek B; Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, 06330, Türkiye., Abaci Kaplan N; Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, 06330, Türkiye., Nazlı Gok H; Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, 06330, Türkiye., Kahraman A; Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Usak University, Usak, 64200, Türkiye., Deliorman Orhan D; Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, 06330, Türkiye., Şenol Deniz FS; Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, 06330, Türkiye., Orhan IE; Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, 06330, Türkiye.; Turkish Academy of Sciences (TÜBA), Vedat Dalokay Cad., No. 112, Ankara, 06670, Türkiye.; Department of Pharmacognosy, Faculty of Pharmacy, Lokman Hekim University, Ankara, 06510, Türkiye.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2024 Oct 07, pp. e202401619. Date of Electronic Publication: 2024 Oct 07.
DOI: 10.1002/cbdv.202401619
Abstrakt: The genera Geranium and Erodium (Geraniaceae) have been documented to possess diverse ethnopharmacological uses, including diabetes mellitus. Relevant to their ethnopharmacological use, the current study aimed to evaluate the α-glucosidase, α-amylase, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) enzyme inhibitory activity of ethanol extracts from 46 samples belonging to thirty-one species of Geranium (20) and Erodium (11) collected throughout Türkiye. The majority of the extracts displayed a marked α-glucosidase and α-amylase inhibitory activity. Besides, 23 extracts out of 46 exhibited a concentration-dependent inhibitory effect over 50 % towards AChE. The highest AChE inhibition was found in G. subcaulescens collected from Konya with an IC 50 value of 4.73±2.96 μg/mL. E. somanum, E. leucanthum, and E. sipthorpianum exhibited the most potent α-glucosidase inhibitory activity, while E. birandianum and E. pelargoniiflorum were the most active extracts against AChE and BChE, respectively. Three extracts that had inhibitory activity over 50 % against four of the enzymes were selected and proceeded to RP-HPLC analysis. Geraniin and ellagic acid were identified as major compounds in the active extracts. Most species screened in the current study were examined for the first time against α-glucosidase, α-amylase, AChE, and BChE.
(© 2024 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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