Mild burn amplifies the locomotive depression in demyelinated mice without muscle pathophysiological changes.

Autor: Song J; Department of Surgery, the University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America., Ei Ayadi A; Department of Surgery, the University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America., Rontoyanni VG; Department of Surgery, the University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America., Wolf SE; Department of Surgery, the University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Oct 07; Vol. 19 (10), pp. e0308908. Date of Electronic Publication: 2024 Oct 07 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0308908
Abstrakt: Introduction: Patients with mild burns take most accounts, however, the impact of mild burns is less known. Nerve destruction leads to muscle atrophy. We posit that even mild burn injury could worsen demyelinated nerves related to muscle pathophysiological impairment.
Methods: Young adult C57BL/6 (male, n = 60) mice were randomly fed with either a 0.2% cuprizone diet or a regular rodent diet for 4 weeks. At week 5, all mice were then grouped into mild scald burn with 10% TBSA and sham injury groups. Mice received animal behavior tests and in situ muscle isometric force measurement before euthanasia for tissue collection.
Results: Total horizontal ambulation and vertical activity were significantly reduced in mice with mild burn injury (p<0.05). Mice with the cuprizone diet had significantly less time to fall than those with the regular diet on day 7 after burn (p<0.05). No significant difference was found in gastrocnemius tissue weight among the groups, nor muscle isometric tensions (all p>0.05). The cuprizone diet increased the maximal phosphorylating respiration in mice muscle mitochondria (p<0.05). The muscle protein expressions of caspase 3, Fbx-32, and Murf1 significantly increased in mice with the cuprizone diet 3 days after burn (p<0.05). The signal expression of S100B significantly increased in mice with the cuprizone diet, and its expression was even greater on day 7 after burn injury. (p<0.05).
Conclusion: The cuprizone diet-induced locomotion and cognitive disorders were amplified by the mild burn injury in mice, which is associated with muscle intracellular signal alterations. However, mild burn injury does not cause mouse muscle weight loss and function impairment. The potential risk of pre-existed neural impairment could be aware when patients encounter even small or mild burns.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Song et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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