| Autor: |
Miller WG; Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, USA., Chapman MH; Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, USA., Williams TG; Bioproducts Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, USA., Wood DF; Bioproducts Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, USA., Bono JL; Meat Safety and Quality Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, NE, USA., Kelly DJ; School of Biosciences, The University of Sheffield, Sheffield, UK. |
| Abstrakt: |
Nine Campylobacter strains were isolated from cattle and feral swine faeces: three were recovered during a 2007 Campylobacter -associated outbreak linked to a dairy, and the other six were isolated during a 2009-2010 survey of farms and ranches in Central California. The species identification of these strains could not be determined by 16S rRNA gene sequencing but were most similar to Campylobacter concisus and Campylobacter mucosalis . Additional atpA typing indicated that the nine strains composed a discrete novel clade related to C. concisus and C. mucosalis . A polyphasic study was undertaken here to clarify their taxonomic position. Phylogenetic analyses were performed based on 16S rRNA gene sequences and the concatenated sequences of 330 core genes. The core gene analysis placed the nine strains into a clade well separated from the other Campylobacter taxa, indicating that these strains represent a novel Campylobacter species. Pairwise digital DNA-DNA hybridization and average nucleotide identity values between these strains and other campylobacters are lower than 16 and 73%, respectively, further supporting their placement into a novel taxon. Standard phenotypic testing was performed. All strains are microaerobic or anaerobic, motile, Gram-negative, slightly-curved rods that are oxidase positive but catalase negative. Strains can be distinguished from the other catalase-negative Campylobacter species using phenotypic markers such as motility, oxidase activity, cephalothin resistance, hippuricase activity, growth at 30 °C, and α-haemolysis. The data presented here show that these strains represent a novel species within Campylobacter , for which the name Campylobacter californiensis sp. nov. (type strain RM6914 T =LMG 32304 T =CCUG 75329 T ) is proposed. |
