Highly Stable Biotemplated InP/ZnSe/ZnS Quantum Dots for In Situ Bacterial Monitoring.

Autor:	Yousefi H; Chan Zuckerberg Biohub Chicago, Chicago, Illinois 60607, United States.; Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, United States., Sagar LK; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, Toronto, Ontario M5S 3G4, Canada., Geraili A; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario M5S 3M2, Canada., Chang D; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, Toronto, Ontario M5S 3G4, Canada.; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario M5S 3M2, Canada., García de Arquer FP; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, Toronto, Ontario M5S 3G4, Canada., Flynn CD; Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3M2, Canada., Lee S; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, Toronto, Ontario M5S 3G4, Canada., Sargent EH; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, Toronto, Ontario M5S 3G4, Canada.; Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Kelley SO; Chan Zuckerberg Biohub Chicago, Chicago, Illinois 60607, United States.; Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, United States.; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario M5S 3M2, Canada.; Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3M2, Canada.; Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States.; Department of Biochemistry and Molecular Genetics, Northwestern University, Chicago, Illinois 60611, United States.
Jazyk:	angličtina
Zdroj:	ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2024 Oct 07. Date of Electronic Publication: 2024 Oct 07.
DOI:	10.1021/acsami.4c09968
Abstrakt:	Despite their unique optical and electrical characteristics, traditional semiconductor quantum dots (QDs) made of heavy metals or carbon are not ideally suited for biomedical applications. Cytotoxicity and environmental concerns are key limiting factors affecting the adoption of QDs from laboratory research to real-world medical applications. Recently, advanced InP/ZnSe/ZnS QDs have emerged as alternatives to traditional QDs due to their low toxicity and optical properties; however, bioconjugation has remained a challenge due to surface chemistry limitations that can lead to instability in aqueous environments. Here, we report water-soluble, biotemplated InP/ZnSe/ZnS-aptamer quantum dots (QDAPTs) with long-term stability and high selectivity for targeting bacterial membrane proteins. QDAPTs show fast binding reaction kinetics (less than 5 min), high brightness, and high levels of stability (3 months) after biotemplating in aqueous solvents. We use these materials to demonstrate the detection of bacterial membrane proteins on common surfaces using a hand-held imaging device, which attests to the potential of this system for biomedical applications.
Databáze:	MEDLINE
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