Multidirectional Filamented Light Biofabrication Creates Aligned and Contractile Cardiac Tissues.

Autor: Jones LS; Soft Robotics Laboratory, ETH Zurich, Tannenstrasse 3, Zurich, 8092, Switzerland., Filippi M; Soft Robotics Laboratory, ETH Zurich, Tannenstrasse 3, Zurich, 8092, Switzerland., Michelis MY; Soft Robotics Laboratory, ETH Zurich, Tannenstrasse 3, Zurich, 8092, Switzerland., Balciunaite A; Soft Robotics Laboratory, ETH Zurich, Tannenstrasse 3, Zurich, 8092, Switzerland., Yasa O; Soft Robotics Laboratory, ETH Zurich, Tannenstrasse 3, Zurich, 8092, Switzerland., Aviel G; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel., Narciso M; Swiss Federal Laboratories for Materials Science and Technology (EMPA), Dubendorf, 8600, Switzerland.; Experimental Continuum Mechanics, ETH Zurich, Leonhardstrasse 21, Zurich, 8092, Switzerland., Freedrich S; ETH Phenomics Center, ETH Zurich, Otto-Stern-Weg 7, Zurich, 8093, Switzerland., Generali M; Institute for Regenerative Medicine (IREM), University of Zurich, Schlieren, 8952, Switzerland., Tzahor E; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel., Katzschmann RK; Soft Robotics Laboratory, ETH Zurich, Tannenstrasse 3, Zurich, 8092, Switzerland.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Oct 07, pp. e2404509. Date of Electronic Publication: 2024 Oct 07.
DOI: 10.1002/advs.202404509
Abstrakt: Biofabricating 3D cardiac tissues that mimic the native myocardial tissue is a pivotal challenge in tissue engineering. In this study, we fabricate 3D cardiac tissues with controlled, multidirectional cellular alignment and directed or twisting contractility. We show that multidirectional filamented light can be used to biofabricate high-density (up to 60 × 10 6 cells mL -1 ) tissues, with directed uniaxial contractility (3.8x) and improved cell-to-cell connectivity (1.6x gap junction expression). Furthermore, by using multidirectional light projection, we can partially overcome cell-induced light attenuation, and fabricate larger tissues with multidirectional cellular alignment. For example, we fabricate a tri-layered myocardium-like tissue and a bi-layered tissue with torsional contractility. The approach provides a new strategy to rapidly fabricate aligned cardiac tissues relevant to regenerative medicine and biohybrid robotics.
(© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
Externí odkaz: