Diverse somatic Transformer and sex chromosome karyotype pathways regulate gene expression in Drosophila gonad development.

Autor:	Mahadevaraju S; Section of Developmental Genomics, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA.; Department of Biology. St. Mary's College of Maryland, St. Mary's City, Maryland, USA., Pal S; National Library of Medicine, National Institutes of Health, Bethesda, Maryland, USA.; Neurobiology Neurodegeneration and Repair Lab, National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA., Bhaskar P; Section of Developmental Genomics, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA., McDonald BD; Section of Developmental Genomics, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA.; Department of Biology, Stanford University, Stanford, California, USA., Benner L; Section of Developmental Genomics, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA., Denti L; Department of Informatics, Systems, and Communication, University of Milano - Bicocca, Milan, Italy., Cozzi D; Department of Informatics, Systems, and Communication, University of Milano - Bicocca, Milan, Italy., Bonizzoni P; Department of Informatics, Systems, and Communication, University of Milano - Bicocca, Milan, Italy., Przytycka TM; National Library of Medicine, National Institutes of Health, Bethesda, Maryland, USA., Oliver B; Section of Developmental Genomics, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Aug 12. Date of Electronic Publication: 2024 Aug 12.
DOI:	10.1101/2024.08.12.607556
Abstrakt:	The somatic sex determination gene transformer ( tra ) is required for the highly sexually dimorphic development of most somatic cells, including those of the gonads. In addition, somatic tra is required for the germline development even though it is not required for sex determination within germ cells. Germ cell autonomous gene expression is also necessary for their sex determination. To understand the interplay between these signals, we compared the phenotype and gene expression of larval wild-type gonads and the sex-transformed tra gonads. XX larval ovaries transformed into testes were dramatically smaller than wild-type, with significant reductions in germ cell number, likely due to altered geometry of the stem cell niche. Additionally, there was a defect in progression into spermatocyte stages. XY larval testes transformed into ovaries had excessive germ cells, possibly due to the earlier onset of cell division. We suggest that germ cells are neither fully female nor male following somatic sex transformation, with certain pathways characteristic of each sex expressed in tra mutants. We found multiple patterns of somatic and germline gene expression control exclusively due to tra , exclusively due to sex chromosome karyotype, but usually due to a combination of these factors showing tra and sex chromosome karyotype pathways regulate gene expression during Drosophila gonad development. Competing Interests: Competing interests The authors declare no competing interest.
Databáze:	MEDLINE
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