Discovery and characterization of novel lipopeptides produced by Virgibacillus massiliensis with biosurfactant and antimicrobial activities.

Autor: Essghaier B; Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University of Tunis Elmanar, 2092 Elmanar II, Tunisia., Naccache C; Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University of Tunis Elmanar, 2092 Elmanar II, Tunisia., Ben-Miled H; Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University of Tunis Elmanar, 2092 Elmanar II, Tunisia., Mottola F; Department of Environmental Biological and Pharmaceutical and Technologies (DiSTABiF), University of Campania L.Vanvitelli-Via Vivaldi, 43-81100 Caserta, Italy., Ben-Mahrez K; Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University of Tunis Elmanar, 2092 Elmanar II, Tunisia., Mezghani Khemakhem M; Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University of Tunis Elmanar, 2092 Elmanar II, Tunisia., Rocco L; Department of Environmental Biological and Pharmaceutical and Technologies (DiSTABiF), University of Campania L.Vanvitelli-Via Vivaldi, 43-81100 Caserta, Italy.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2024 Nov; Vol. 14 (11), pp. 258. Date of Electronic Publication: 2024 Oct 04.
DOI: 10.1007/s13205-024-04100-9
Abstrakt: The study aimed to evaluate the biosurfactants (BSs) production by SM-23 strain of Virgibacillus identified by phenotypical and WGS analysis as Virgibacillus massiliensis . We first demonstrated the lipopeptides production by Virgibacillus massiliensis specie and studied their biochemical and molecular analysis as well as their biological potential. The GC-MS analysis indicated that methyl.2-hyroxydodecanoate was the major fatty acid compound with 33.22%. The maximum BSs production was obtained in LB medium supplemented by 1% olive oil (v/v) at 30 °C and 5% NaCl with 1.92 g/l. The obtained results revealed the significant biosurfactants/bioemulsifier potential compared to triton X100 with E24 of 100%, and an emulsification stability SE of 83%. The lipopeptides types were identified by FTIR analysis. A strong antimicrobial action was observed by the produced lipopeptides by the agar diffusion method against E.coli, K. pneumoniae, S. aureus, Fusarium sp, Alternaria sp, and Phytophtora sp. The complete genome sequencing showed genes involved in the synthesis of multiple compounds identified as amphipathic cyclic lipopeptides such as locillomycin/locillomycin B/locillomycin C and bacillibactin. Our results highlighted significant lipopeptides properties displayed by V. massiliensis that can be exploited to develop a novel strategy in the formulation of natural biocidal and fungicidal agents.
Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-024-04100-9.
Competing Interests: Conflict of interestOn behalf of all authors, the corresponding author states that there is no conflict of interest.
(© The Author(s) 2024.)
Databáze: MEDLINE
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