| Autor: |
Schodt DJ; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Wester MJ; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America.; Department of Mathematics and Statistics, Albuquerque, University of New Mexico, United States of America., Fazel M; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Khan S; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Mazloom-Farsibaf H; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Pallikkuth S; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Meddens MBM; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Farzam F; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Burns EA; Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, United States of America., Kanagy WK; Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, United States of America., Rinaldi DA; Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, United States of America., Jhamba E; Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, United States of America., Liu S; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Relich PK; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Olah MJ; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America., Steinberg SL; Department of Mathematics and Statistics, Albuquerque, University of New Mexico, United States of America., Lidke KA; Department of Physics and Astronomy, University of New Mexico, Albuquerque, United States of America. |
| Abstrakt: |
Fluorescence single molecule imaging comprises a variety of techniques that involve detecting individual fluorescent molecules. Many of these techniques involve localizing individual fluorescent molecules with precisions below the diffraction limit, which limits the spatial resolution of (visible) light-based microscopes. These methodologies are widely used to image biological structures at the nanometer scale by fluorescently tagging the structures of interest, elucidating details of the biological behavior observed. Two common techniques are single-molecule localization microscopy (SMLM), (Betzig et al., 2006; Fazel & Wester, 2022; Hell, 2007; Lidke et al., 2005; Rust et al., 2006; van de Linde et al., 2011) which is used to produce 2D or 3D super-resolution images of static or nearly static structures, and single-particle tracking (SPT) (Shen et al., 2017), which follows the time course of one or a very small number of moving tagged molecules. SMLM often involves distributions of particles at medium to high density, while SPT works in a very low density domain. These procedures all require intensive numerical computation, and the methods are tightly interwoven. |
