From the discovery of a novel arepavirus in diseased arecanut palms (Areca catechu L.) in India to the identification of known and novel arepaviruses in bee and plant transcriptomes through data-mining.

Autor: Thava Prakasa Pandian R; ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI) Regional Station, Vittal, Karnataka, India., Bhavishya; ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI) Regional Station, Vittal, Karnataka, India., Kavi Sidharthan V; Division of Genetics and Tree Improvement, ICFRE-Institute of Forest Biodiversity (IFB), Hyderabad, India., Rajesh MK; ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI) Regional Station, Vittal, Karnataka, India. Electronic address: rajesh.mk@icar.gov.in., Babu M; ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI) Regional Station, Kayamkulam, Kerala, India., Sharma SK; ICAR-Indian Agricultural Research Institute (ICAR-IARI), New Delhi, India., Nirmal Kumar BJ; ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI) Regional Station, Vittal, Karnataka, India., Chaithra M; ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI) Research Centre, Kakikuchi, Assam, India., Hegde V; ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI) Kasaragod, Kerala, India.
Jazyk: angličtina
Zdroj: Virology [Virology] 2024 Dec; Vol. 600, pp. 110256. Date of Electronic Publication: 2024 Oct 02.
DOI: 10.1016/j.virol.2024.110256
Abstrakt: Arecanut palm is a commercially important plantation crop valued for its nut. In this investigation, we report the discovery of a putative novel arepavirus, named areca palm necrotic ringspot virus 2 (ANRSV2), in necrotic ringspot diseased areca palms in Bantwal, Dakshina Kannada, Karnataka, India through RNA-sequencing and transmission electron microscopy. Further, the presence of ANRSV2 in the diseased samples was confirmed through reverse transcriptase-polymerase chain reaction assays. In addition, by mining public domain transcriptome data for arepaviral sequences, we identified a putative novel arepavirus in Psychotria rubra, a non-palm host. We recovered the genome sequences of the areca palm necrotic ringspot virus in honey bees, tomato, Onobrychis viciifolia, and Rhamnus heterophylla. These findings broaden our comprehension of arepaviral diversity and host range, and suggest an intriguing possibility of pollen-mediated arepaviral transmission that necessitates empirical validation. Further studies are needed to understand the biology of identified putative novel arepaviruses.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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