Soft surface-enhanced Raman scattering sensing platform based on an oil-in-water emulsion stabilized by silver nanoparticles.

Autor: Klein H; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, 68 HaMaccabim Road, Rishon Letzion 7505101, Israel; School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Ein Karem, Jerusalem 9112102, Israel., Cohen R; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, 68 HaMaccabim Road, Rishon Letzion 7505101, Israel; Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 7610001, Israel., Mani KA; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, 68 HaMaccabim Road, Rishon Letzion 7505101, Israel; Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 7610001, Israel., Feldbaum RA; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, 68 HaMaccabim Road, Rishon Letzion 7505101, Israel., Ben-Haim A; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, 68 HaMaccabim Road, Rishon Letzion 7505101, Israel; Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 7610001, Israel., Zelinger E; Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 7610001, Israel., Nirala NR; Institute of Agricultural Engineering, ARO, Volcani Institute, Bet Dagan 50250, Israel., Muthukumar D; Institute of Agricultural Engineering, ARO, Volcani Institute, Bet Dagan 50250, Israel., Domb AJ; School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Ein Karem, Jerusalem 9112102, Israel., Shtenberg G; Institute of Agricultural Engineering, ARO, Volcani Institute, Bet Dagan 50250, Israel., Mechrez G; Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Institute, 68 HaMaccabim Road, Rishon Letzion 7505101, Israel. Electronic address: guyme@agri.gov.il.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2025 Jan; Vol. 245, pp. 114278. Date of Electronic Publication: 2024 Sep 30.
DOI: 10.1016/j.colsurfb.2024.114278
Abstrakt: Pickering emulsions are excellent candidates for developing soft biosensors utilized for detecting native biomolecules such as peptides and proteins through the Surface-Enhanced Raman Spectroscopy (SERS) transduction mechanism. Here, we have developed a SERS sensor based on oil-in-water Pickering emulsions stabilized by Ag nanoparticles (Ag-NPs) with the Raman active molecule (4-Aminothiphenol, 4ATP) adsorbed to their surface. The structural properties and composition of the Pickering emulsion were tuned to meet the demands of the maximal optical response. Our results show that the obtained SERS signals of the main studied Pickering emulsion (water: oil ratio 7:3, 1 wt% Ag-NPs) outperformed colloidal dispersions with the same Ag-NPs concentration by 10-fold at any studied content of 4ATP. The superior optical response of the Pickering emulsion compared to the colloidal dispersion can thus pave the way for the detection of a large variety of analytes at high sensitivity by a soft sensing device. This study innovates by comparing the SERS signals of Raman-active Ag-NPs when they are assembled at the oil/water interface of an emulsion to the case where the NPs are individually dispersed in the medium. The findings shed light on the edit value of utilizing Raman-active Pickering stabilizers for biosensing applications.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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