Induction of immunogenic cell death and enhancement of the radiation-induced immunogenicity by chrysin in melanoma cancer cells.

Autor: Jafari S; Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Ardakan AK; Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran., Aghdam EM; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran.; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran., Mesbahi A; Medical Radiation Research Team, 84 Gorge Road, South Morang, Melbourne, Australia., Montazersaheb S; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran. smontazersaheb@gmail.com., Molavi O; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran. molavio@tbzmed.ac.ir.; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran. molavio@tbzmed.ac.ir.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 05; Vol. 14 (1), pp. 23231. Date of Electronic Publication: 2024 Oct 05.
DOI: 10.1038/s41598-024-72697-1
Abstrakt: Chrysin is a natural flavonoid with anti-cancer effects. Despite its beneficial effects, little information is available regarding its immunogenic cell death (ICD) properties. In this work, we hypothesized that chrysin can potentiate radiotherapy(RT)-induced immunogenicity in melanoma cell line (B16-F10). We examined the effects of chrysin alone and in combination with radiation on ICD induction in B16-F10 cells. Cell viability was assessed using an MTT assay. Cell apoptosis and calreticulin (CRT) exposure were determined using flow cytometry. Western blotting and ELISA assay were employed to examine changes in protein expression. Combination therapy exhibited a synergistic effect, with an optimum combination index of 0.66. The synergistic anti-cancer effect correlated with increased cell apoptosis in cancer cells. Compared to the untreated control, chrysin alone and in combination with RT induced higher levels of DAMPs, such as CRT, HSP70, HMGB1, and ATP. The protein expression of p-STAT3/STAT3 and PD-L1 was reduced in B16-F10 cells exposed to chrysin alone and in combination with RT. Conditioned media from B16-F10 cells exposed to mono-and combination treatments elicited IL-12 secretion in dendritic cells (DCs), inducing a Th1 response. Our findings revealed that chrysin could induce ICD and intensify the RT-induced immunogenicity.
(© 2024. The Author(s).)
Databáze: MEDLINE
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