Combining human liver ECM with topographically featured electrospun scaffolds for engineering hepatic microenvironment.

Autor: Gao Y; Institute for Bioengineering, School of Engineering, University of Edinburgh, Edinburgh, UK.; Foundation of Liver Research, The Roger Williams Institute of Liver Study, London, UK., Gadd VL; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh, UK., Heim M; Institute for Bioengineering, School of Engineering, University of Edinburgh, Edinburgh, UK.; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh, UK., Grant R; MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, The Netherlands., Bate TSR; Institute for Bioengineering, School of Engineering, University of Edinburgh, Edinburgh, UK.; Vanderbilt University Medical Center, Nashville, USA., Esser H; Institute for Bioengineering, School of Engineering, University of Edinburgh, Edinburgh, UK.; Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, Austria., Gonzalez SF; Centre for Inflammation Research, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh, UK., Man TY; Centre for Inflammation Research, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh, UK., Forbes SJ; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh, UK., Callanan A; Institute for Bioengineering, School of Engineering, University of Edinburgh, Edinburgh, UK. Anthony.Callanan@ed.ac.uk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 05; Vol. 14 (1), pp. 23192. Date of Electronic Publication: 2024 Oct 05.
DOI: 10.1038/s41598-024-73827-5
Abstrakt: Liver disease cases are rapidly expanding worldwide, and transplantation remains the only effective cure for end-stage disease. There is an increasing demand for developing potential drug treatments, and regenerative therapies using in-vitro culture platforms. Human decellularized extracellular matrix (dECM) is an appealing alternative to conventional animal tissues as it contains human-specific proteins and can serve as scaffolding materials. Herein we exploit this with human donor tissue from discarded liver which was not suitable for transplant using a synergistic approach to combining biological and topographical cues in electrospun materials as an in-vitro culture platform. To realise this, we developed a methodology for incorporating human liver dECM into electrospun polycaprolactone (PCL) fibres with surface nanotopographies (230-580 nm). The hybrid scaffolds were fabricated using varying concentrations of dECM; their morphology, mechanical properties, hydrophilicity and stability were analysed. The scaffolds were validated using HepG2 and primary mouse hepatocytes, with subsequent results indicating that the modified scaffolds-maintained cell growth and influenced cell attachment, proliferation and hepatic-related gene expression. This work demonstrates a novel approach to harvesting the potential from decellularized human tissues in the form of innovative in-vitro culture platforms for liver.
(© 2024. The Author(s).)
Databáze: MEDLINE
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