An impedimetric determination of zearalenone on MIP-modified carboceramic electrode.

Autor: Küçük D; Kutahya Dumlupınar University, Chemistry Department, Kütahya, Turkey., Üner G; Kutahya Dumlupınar University, Chemistry Department, Kütahya, Turkey., İpek SL; Kutahya Dumlupınar University, Chemistry Department, Kütahya, Turkey; Adana Alparslan Türkeş Science and Technology University, Department of Food Eng., Adana, Turkey. Electronic address: slipek@atu.edu.tr., Caglayan MO; Bilecik Şeyh Edebali University, Bioengineering Department, Bilecik, Turkey., Üstündağ Z; Kutahya Dumlupınar University, Chemistry Department, Kütahya, Turkey. Electronic address: zustundag@gmail.com.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 Nov 06; Vol. 250, pp. 108115. Date of Electronic Publication: 2024 Oct 03.
DOI: 10.1016/j.toxicon.2024.108115
Abstrakt: Zearalenone (ZEN) is a mycotoxin that poses significant risks to human and animal health due to its mutagenic, immunosuppressive, and carcinogenic properties. This study presents a novel analytical method for detecting ZEN using electrochemical impedance spectroscopy (EIS) combined with a molecularly imprinted polymer (MIP). ZEN, used as the template molecule, was incorporated into polypyrrole on screen-printed electrodes (SPE), and a ZEN-sensitive MIP sensor was created through template removal. The modified sensor surfaces were characterized by EIS and scanning electron microscopy (SEM). An impedimetric MIP sensor for ZEN was developed, offering a detection range from 1 pM to 500 pM. The method's limit of detection (LOD) was established at 1 pM (0.3 pg/mL) with a signal-to-noise ratio of 3 (S/N = 3). The method demonstrated high precision and accuracy, with a maximum relative standard deviation (RSD) of less than 4.4% at a 95% confidence level, and relative error (RE) values ranging from -0.8% to -2.7%. The selectivity of the developed MIP sensor was evaluated using ochratoxin A, ochratoxin B, and aflatoxin B1, with no significant interference observed. ZEN recovery from spiked samples was between 95% and 105%, indicating that the method was successfully applied to grain samples, including corn, rice, and wheat.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: