HCN channel-dependent presynaptic potentiation at LA-BA synapses is required for fear memory formation.

Autor: Choi K; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea., Yi JH; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea., Park K; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea., Woo C; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea., Lee C; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea., Kang SJ; Department of Integrative Bioscience and Biotechnology, Sejong University, Seoul, 05006, Republic of Korea., Shin KS; Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea. Electronic address: kisoon_shin@khu.ac.kr.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Nov 19; Vol. 734, pp. 150788. Date of Electronic Publication: 2024 Oct 02.
DOI: 10.1016/j.bbrc.2024.150788
Abstrakt: Previously, we demonstrated that auditory fear conditioning produces presynaptic potentiation at lateral to basal amygdala (LA-BA) synapses, which occludes high-frequency stimulation (HFS)-induced ex-vivo LTP. We also found that the HFS-induced ex-vivo LTP requires presynaptic hyperpolarization-activated cyclic nucleotide-gated (HCN) channel activity. In this study, we investigated whether HCN channels are necessary for auditory fear conditioning in vivo. Our results show that ZD7288, an HCN channel blocker, reduced synaptic transmission and decreased the paired pulse ratio (PPR) only in slices from rats that underwent auditory fear conditioning, but not from naïve rats. This indicates that fear conditioning involves HCN channel-dependent presynaptic potentiation at LA-BA synapses. Importantly, injecting ZD7288 into the basal amygdala (BA) before auditory fear conditioning significantly impaired long-term fear memory formation. Since HCN channel activity is necessary for LTP at LA-BA synapses but not at cortico-BA, cortico-LA, or thalamo-LA synapses, HCN channel-dependent presynaptic potentiation at LA-BA synapses appears to be crucial for auditory fear conditioning.
Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be constructed as a potential conflict of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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