Unveiling placental development in circadian rhythm-disrupted mice: A photo-acoustic imaging study on unstained tissue.

Autor: Cizmeciyan MN; Department of Physics, Bogazici University, Bebek, Istanbul, 34342, Turkey; Faculty of Engineering, Ozyegin University, Cekmekoy, Istanbul, 34794, Turkey., Bektas NI; Department of Histology and Embryology, School of Medicine, Akdeniz University, Antalya, Turkey., Derin N; Department of Biophysics, School of Medicine, Akdeniz University, Antalya, Turkey., Denizaltı T; Department of Biophysics, School of Medicine, Akdeniz University, Antalya, Turkey., Khoshzaban A; Department of Physics, Bogazici University, Bebek, Istanbul, 34342, Turkey., Unlu MB; Department of Physics, Bogazici University, Bebek, Istanbul, 34342, Turkey; Faculty of Engineering, Ozyegin University, Cekmekoy, Istanbul, 34794, Turkey., Celik-Ozenci C; Department of Histology and Embryology, School of Medicine, Koc University, Rumelifeneri, Sariyer, Istanbul, 34450, Turkey; Koc University Research Center for Translational Medicine (KUTTAM), Koc University, Istanbul, Turkey. Electronic address: cozenci@ku.edu.tr.
Jazyk: angličtina
Zdroj: Placenta [Placenta] 2024 Dec; Vol. 158, pp. 57-61. Date of Electronic Publication: 2024 Oct 02.
DOI: 10.1016/j.placenta.2024.10.001
Abstrakt: Introduction: Circadian rhythm disruption has garnered significant attention for its adverse effects on human health, particularly in reproductive medicine and fetal well-being. Assessing pregnancy health often relies on diagnostic markers such as the labyrinth zone (LZ) proportion within the placenta. This study aimed to investigate the impact of disrupted circadian rhythms on placental health and fetal development using animal models.
Methods and Results: Employing unstained photo-acoustic microscopy (PAM) and hematoxylin and eosin (HE)-stained images, we found them mutually reinforcing. Our images revealed the role of maternal circadian rhythm disrupted group (MCRD) on the LZ and fetus weight: a decrease in LZ area from 5.01 (4.25) mm 2 HE (PAM) to 3.58 (2.62) mm 2 HE (PAM) on day 16 and 6.48 (5.16) mm 2 HE (PAM) to 4.61 (3.03) mm 2 HE (PAM) on day 18, resulting in 0.71 times lower fetus weights. We have discriminated a decrease in the mean LZ to placenta area ratio from 64 % to 47 % on day 18 in mice with disrupted circadian rhythms with PAM.
Discussion: The study highlights the negative influence of circadian rhythm disruption on placental development and fetal well-being. Reduced LZ area and fetal weights in the MCRD group suggest compromised placental function under disrupted circadian rhythms. PAM imaging proved to be an efficient technique for assessing placental development, offering advantages over traditional staining methods. These findings contribute to understanding the underlying mechanisms of circadian disruption on reproductive health and fetal development. Further research is needed to explore interventions to mitigate these effects and improve pregnancy outcomes.
Competing Interests: Declaration of competing interest None.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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