An atlas of small non-coding RNAs in human preimplantation development.

Autor: Russell SJ; CReATe Fertility Centre, Toronto, ON, Canada., Zhao C; Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden.; Division of Obstetrics and Gynecology, Karolinska Universitetssjukhuset, Stockholm, Sweden., Biondic S; Faculty of Medicine, Molecular Biology Program, Université de Montréal, Montréal, QC, Canada.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Axe Immunopathologie, Montréal, Canada., Menezes K; CReATe Fertility Centre, Toronto, ON, Canada., Hagemann-Jensen M; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77, Stockholm, Sweden., Librach CL; CReATe Fertility Centre, Toronto, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.; Department of Obstetrics and Gynaecology, University of Toronto, Toronto, ON, Canada.; Department of Physiology, University of Toronto, Toronto, ON, Canada.; Sunnybrook Research Institute, Toronto, ON, Canada.; Institute of Medical Sciences, University of Toronto, Toronto, ON, Canada., Petropoulos S; Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden. sophie.petropoulos@umontreal.ca.; Division of Obstetrics and Gynecology, Karolinska Universitetssjukhuset, Stockholm, Sweden. sophie.petropoulos@umontreal.ca.; Faculty of Medicine, Molecular Biology Program, Université de Montréal, Montréal, QC, Canada. sophie.petropoulos@umontreal.ca.; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Axe Immunopathologie, Montréal, Canada. sophie.petropoulos@umontreal.ca.; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77, Stockholm, Sweden. sophie.petropoulos@umontreal.ca.; Faculty of Medicine, Département de Médecine, Université de Montréal, Montréal, QC, Canada. sophie.petropoulos@umontreal.ca.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Oct 05; Vol. 15 (1), pp. 8634. Date of Electronic Publication: 2024 Oct 05.
DOI: 10.1038/s41467-024-52943-w
Abstrakt: Understanding the molecular circuitries that govern early embryogenesis is important, yet our knowledge of these in human preimplantation development remains limited. Small non-coding RNAs (sncRNAs) can regulate gene expression and thus impact blastocyst formation, however, the expression of specific biotypes and their dynamics during preimplantation development remains unknown. Here we identify the abundance of and kinetics of piRNA, rRNA, snoRNA, tRNA, and miRNA from embryonic day (E)3-7 and isolate specific miRNAs and snoRNAs of particular importance in blastocyst formation and pluripotency. These sncRNAs correspond to specific genomic hotspots: an enrichment of the chromosome 19 miRNA cluster (C19MC) in the trophectoderm (TE), and the chromosome 14 miRNA cluster (C14MC) and MEG8-related snoRNAs in the inner cell mass (ICM), which may serve as 'master regulators' of potency and lineage. Additionally, we observe a developmental transition with 21 isomiRs and in tRNA fragment (tRF) codon usage and identify two novel miRNAs. Our analysis provides a comprehensive measure of sncRNA biotypes and their corresponding dynamics throughout human preimplantation development, providing an extensive resource. Better understanding the sncRNA regulatory programmes in human embryogenesis will inform strategies to improve embryo development and outcomes of assisted reproductive technologies. We anticipate broad usage of our data as a resource for studies aimed at understanding embryogenesis, optimising stem cell-based models, assisted reproductive technology, and stem cell biology.
(© 2024. The Author(s).)
Databáze: MEDLINE
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