Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging.

Autor: Milicevic KD; School of Medicine, Institute for Systems Genomics, UConn Health, University of Connecticut Health, 263 Farmington Avenue, Farmington, CT, 06030, USA.; Institute of Physiology and Biochemistry 'Jean Giaja', Center for Laser Microscopy, University of Belgrade, Faculty of Biology, 11000, Belgrade, Serbia., Ivanova VO; School of Medicine, Institute for Systems Genomics, UConn Health, University of Connecticut Health, 263 Farmington Avenue, Farmington, CT, 06030, USA., Lovic DD; School of Medicine, Institute for Systems Genomics, UConn Health, University of Connecticut Health, 263 Farmington Avenue, Farmington, CT, 06030, USA.; Institute of Physiology and Biochemistry 'Jean Giaja', Center for Laser Microscopy, University of Belgrade, Faculty of Biology, 11000, Belgrade, Serbia., Platisa J; The John B. Pierce Laboratory, New Haven, CT, 06519, USA.; Department of Cellular and Molecular Physiology, School of Medicine, Yale University, New Haven, CT, 06519, USA., Andjus PR; Institute of Physiology and Biochemistry 'Jean Giaja', Center for Laser Microscopy, University of Belgrade, Faculty of Biology, 11000, Belgrade, Serbia., Antic SD; School of Medicine, Institute for Systems Genomics, UConn Health, University of Connecticut Health, 263 Farmington Avenue, Farmington, CT, 06030, USA. antic@uchc.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 04; Vol. 14 (1), pp. 22787. Date of Electronic Publication: 2024 Oct 04.
DOI: 10.1038/s41598-024-70319-4
Abstrakt: In calcium imaging studies, Ca 2+ transients are commonly interpreted as neuronal action potentials (APs). However, our findings demonstrate that robust optical Ca 2+ transients primarily stem from complex "AP-Plateaus", while simple APs lacking underlying depolarization envelopes produce much weaker photonic signatures. Under challenging in vivo conditions, these "AP-Plateaus" are likely to surpass noise levels, thus dominating the Ca 2+ recordings. In spontaneously active neuronal culture, optical Ca 2+ transients (OGB1-AM, GCaMP6f) exhibited approximately tenfold greater amplitude and twofold longer half-width compared to optical voltage transients (ArcLightD). The amplitude of the ArcLightD signal exhibited a strong correlation with the duration of the underlying membrane depolarization, and a weaker correlation with the presence of a fast sodium AP. Specifically, ArcLightD exhibited robust responsiveness to the slow "foot" but not the fast "trunk" of the neuronal AP. Particularly potent stimulators of optical signals in both Ca 2+ and voltage imaging modalities were APs combined with plateau potentials (AP-Plateaus), resembling dendritic Ca 2+ spikes or "UP states" in pyramidal neurons. Interestingly, even the spikeless plateaus (amplitude > 10 mV, duration > 200 ms) could generate conspicuous Ca 2+ optical signals in neurons. Therefore, in certain circumstances, Ca 2+ transients should not be interpreted solely as indicators of neuronal AP firing.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje